Goldberg I J, Paterniti J R, Brown W V
Biochim Biophys Acta. 1983 Jun 16;752(1):172-7. doi: 10.1016/0005-2760(83)90245-x.
Conditions for measurement of the lipolytic activities, lipoprotein lipase and hepatic triacylglycerol lipase in cynomolgus monkey postheparin plasma are described. The two activities are separable by heparin-Sepharose chromatography. Goat anti-human hepatic triacylglycerol lipase serum inhibits monkey hepatic triacylglycerol lipase activity and allows direct measurement of lipoprotein lipase in post-heparin plasma. While both human and homologous serum can be used as a source of activator apolipoprotein, homologous serum produces a much greater activation.
描述了食蟹猴肝素后血浆中脂解活性、脂蛋白脂肪酶和肝三酰甘油脂肪酶的测量条件。这两种活性可通过肝素-琼脂糖凝胶色谱法分离。山羊抗人肝三酰甘油脂肪酶血清可抑制猴肝三酰甘油脂肪酶活性,并可直接测量肝素后血浆中的脂蛋白脂肪酶。虽然人血清和同源血清都可作为激活载脂蛋白的来源,但同源血清产生的激活作用要强得多。