两栖动物核糖体核糖核酸
Amphibian ribosomal ribonucleic acids.
作者信息
Rogers M E, Klein G
出版信息
Biochem J. 1972 Nov;130(1):281-8. doi: 10.1042/bj1300281.
Abstract
- The larger rRNA molecules of different species of amphibia do not all have the same mobility on polyacrylamide gels, and the difference appears to be one between the Anuran and Urodele amphibia. 2. Alteration of conditions of electrophoresis so as to distinguish between differences of conformation and molecular weight does not affect the results. 3. The mobility on gel electrophoresis of the rRNA precursor in two species of amphibia is similar, although the intermediate processing products differ. 4. It is suggested that the mobility differences observed represent differences of molecular weight, and are an example of the variability of the products of transcription and processing of rRNA genes in two amphibian genera.
摘要
- 不同种类两栖动物的较大核糖体RNA分子在聚丙烯酰胺凝胶上的迁移率并非都相同，这种差异似乎存在于无尾两栖类和有尾两栖类之间。2. 改变电泳条件以区分构象差异和分子量差异并不会影响结果。3. 两种两栖动物中核糖体RNA前体在凝胶电泳上的迁移率相似，尽管中间加工产物有所不同。4. 有人提出，观察到的迁移率差异代表分子量差异，是两个两栖类属中核糖体RNA基因转录和加工产物变异性的一个例子。
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