Tiollais P, Galibert F, Boiron M
Proc Natl Acad Sci U S A. 1971 Jun;68(6):1117-20. doi: 10.1073/pnas.68.6.1117.
Analysis of RNA (after 5 or 60 min of labeling with [(3)H]uridine) from L5178Y cells by electrophoresis in 1.7% polyacrylamide gels demonstrates that the ribosomal RNA "45S fraction" is not homogeneous but consists of at least three molecular species, conventionally designated 47S RNA, 46S RNA, and 45S RNA. Alternatives to the classical scheme for the biosynthesis of ribosomal RNA in mammalian cells are discussed.
用[³H]尿苷标记5分钟或60分钟后,对L5178Y细胞的RNA进行1.7%聚丙烯酰胺凝胶电泳分析表明,核糖体RNA“45S组分”并非单一成分,而是至少由三种分子种类组成,传统上分别称为47S RNA、46S RNA和45S RNA。文中还讨论了哺乳动物细胞核糖体RNA生物合成经典途径的替代方案。