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相似文献

1
Measurement of DNA synthesis in leucocyte microcultures.白细胞微量培养物中DNA合成的测量。
J Clin Pathol. 1972 Dec;25(12):1075-8. doi: 10.1136/jcp.25.12.1075.
2
The millipore filter assay technique for measuring tritiated thymidine incorporation into DNA in leucocyte cultures.用于测量氚标记胸腺嘧啶核苷掺入白细胞培养物中DNA的微孔滤膜检测技术。
Clin Exp Immunol. 1972 Aug;11(4):629-40.
3
Factors affecting the reactivity of human lymphocytes in vitro. I. Cell number, duration of culture and surface area.体外影响人淋巴细胞反应性的因素。I. 细胞数量、培养持续时间和表面积。
J Immunol. 1967 Aug;99(2):413-9.
4
Rapid and sensitive assay for D.N.A. synthesis in leucocyte cultures.白细胞培养中DNA合成的快速灵敏检测法。
Lancet. 1970 Jul 11;1(7663):98. doi: 10.1016/s0140-6736(70)92667-x.
5
'Delayed' peaks of DNA synthesis in phytohemagglutinin-stimulated human leucocyte cultures.植物血凝素刺激的人白细胞培养物中DNA合成的“延迟”峰值
Int Arch Allergy Appl Immunol. 1970;39(5-6):580-6. doi: 10.1159/000230383.
6
DNA and RNA synthesis and the formation of blastogenic factor in mixed leucocyte cultures.
Nature. 1967 Jul 1;215(5096):80-1. doi: 10.1038/215080a0.
7
Differential sensitivity of antigen- and mitogen-stimulated human leucocytes to prolonged inhibition of potassium transport.抗原和丝裂原刺激的人白细胞对钾转运长期抑制的差异敏感性。
Exp Eye Res. 1973 Apr;79(1):87-94.
8
Differential sensitivity of antigen- and mitogen-stimulated human leukocytes to prolonged inhibition of potassium transport.抗原和丝裂原刺激的人白细胞对钾转运长期抑制的差异敏感性。
Exp Cell Res. 1973 Apr;79(1):87-94.
9
Alternative pathways for DNA-thymine synthesis in stimulated leucocytes.刺激后白细胞中DNA胸腺嘧啶合成的替代途径。
Clin Exp Immunol. 1971 Feb;8(2):177-86.
10
Incorporation of 3H-5-uridine by human peripheral mononuclear leucocytes changing from the non-multiplying to the multiplying state.
Exp Cell Res. 1966 Aug;43(1):84-94. doi: 10.1016/0014-4827(66)90381-8.

本文引用的文献

1
The millipore filter assay technique for measuring tritiated thymidine incorporation into DNA in leucocyte cultures.用于测量氚标记胸腺嘧啶核苷掺入白细胞培养物中DNA的微孔滤膜检测技术。
Clin Exp Immunol. 1972 Aug;11(4):629-40.
2
Rapid and sensitive assay for D.N.A. synthesis in leucocyte cultures.白细胞培养中DNA合成的快速灵敏检测法。
Lancet. 1970 Jul 11;1(7663):98. doi: 10.1016/s0140-6736(70)92667-x.

白细胞微量培养物中DNA合成的测量。

Measurement of DNA synthesis in leucocyte microcultures.

作者信息

Robbins J H

出版信息

J Clin Pathol. 1972 Dec;25(12):1075-8. doi: 10.1136/jcp.25.12.1075.

DOI:10.1136/jcp.25.12.1075
PMID:4655859
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC477624/
Abstract

A microculture technique is described in which human peripheral blood leucocytes undergo DNA synthesis in response to specific and non-specific blastogenic factors. A sensitive Millipore filter assay technique is shown to be suitable for determining the incorporation of radioactive thymidine into the DNA of the stimulated cells in these microcultures. With these methods it is possible to measure the DNA synthesis in a 50-mul culture containing an innoculum of only 10(5) leucocytes. This small culture volume, with its low number of leucocytes, therefore makes it possible to perform experiments when either the available leucocytes or blastogenic factors are in limited supply.

摘要

本文描述了一种微培养技术，其中人类外周血白细胞可响应特异性和非特异性致有丝分裂因子进行DNA合成。一种灵敏的微孔滤膜检测技术被证明适用于测定这些微培养中放射性胸苷掺入受刺激细胞DNA的情况。通过这些方法，能够在仅含有10⁵个白细胞接种量的50微升培养物中测量DNA合成。因此，这种少量白细胞的小培养体积使得在可用白细胞或致有丝分裂因子供应有限时进行实验成为可能。