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[来自米曲霉的核酸酶,对核酸的单链区域具有特异性]

[Nuclease from Aspergillus oryzae, specific for single-stranded regions of nucleic acids].

作者信息

Abramov R E, Bezirdzhian Kh O, Akopian Zh I

出版信息

Biokhimiia. 1979 Jun;44(6):990-5.

PMID:465607
Abstract

A single-stranded specific nuclease has been purified from amyloryzine obtained from the mould fungi Aspergillus cryzae. The nuclease under study resembles the enzymes described in the literature in its ability to hydrolyze single-stranded nucleic acids. However, the enzyme essentially differs from previously known nucleases in some catalytic properties, particularly in its ability for degradation of poly A. It has been shown that the enzyme also hydrolyzes the synthetic dinucleotide pTpT to mononucleoside phosphates.

摘要

从米曲霉霉菌的淀粉酶中纯化出了一种单链特异性核酸酶。所研究的这种核酸酶在水解单链核酸的能力方面与文献中描述的酶相似。然而,该酶在一些催化特性上与先前已知的核酸酶有本质区别,特别是在其降解多聚A的能力方面。已经表明,该酶还能将合成二核苷酸pTpT水解为单核苷磷酸。

相似文献

1
[Nuclease from Aspergillus oryzae, specific for single-stranded regions of nucleic acids].[来自米曲霉的核酸酶,对核酸的单链区域具有特异性]
Biokhimiia. 1979 Jun;44(6):990-5.
2
[Isolation and characteristics of highly purified S1-nuclease from amylorizin P10X].[从支链淀粉酶P10X中分离出高纯度S1核酸酶及其特性]
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S1 nuclease of Aspergillus oryzae.米曲霉的S1核酸酶。
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Purification and properties of S1 nuclease from Aspergillus.来自曲霉的S1核酸酶的纯化及性质
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S1 nuclease of Aspergillus oryzae: a glycoprotein with an associated nucleotidase activity.米曲霉S1核酸酶:一种具有相关核苷酸酶活性的糖蛋白。
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Specificity of the S1 nuclease from Aspergillus oryzae.米曲霉S1核酸酶的特异性
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S1 nuclease of Aspergillus oryzae: characterization of the associated phosphomonoesterase activity.米曲霉S1核酸酶:相关磷酸单酯酶活性的表征
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Immobilization of single-strand specific nuclease (S1 nuclease) from Aspergillus oryzae.米曲霉单链特异性核酸酶(S1核酸酶)的固定化。
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