Zagrebel'nyĭ S N, Kamynina T P, Pustoshilova N M, Rivkin M E
Prikl Biokhim Mikrobiol. 1981 Jan-Feb;17(1):113-20.
S1-nuclease was purified from the Soviet commercial enzyme amylorizin P10x prepared from the surface culture of Aspergillus oryzae. The enzyme yield was 33% of total activity. The molecular weight of the enzyme measured by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate was equal to 30,000. The enzyme showed high specificity to single-stranded DNA.
S1核酸酶是从苏联市售的由米曲霉表面培养物制备的淀粉酶P10x中纯化得到的。该酶的产率为总活性的33%。在十二烷基硫酸钠存在下通过聚丙烯酰胺凝胶电泳测定的该酶分子量等于30,000。该酶对单链DNA表现出高特异性。
