Erno H
Infect Immun. 1972 Jan;5(1):20-3. doi: 10.1128/iai.5.1.20-23.1972.
An investigation was carried out to determine the extent to which serology may contribute a means of elucidating the possible etiological significance of the presence of Mycoplasma bovigenitalium in bulls with genital tract disease. Experimentally infected bulls showed a significant serological response with maximum titers of antibody as early as 12 days after inoculation as measured by the indirect hemagglutination test. The tetrazolium reduction inhibition test, even as modified, was less suitable because this method did not reveal antibodies in all inoculated animals. The indirect hemagglutination test revealed high titers of antibody in serum of most bulls from bull stations in Denmark and Luxembourg although young bulls were often serologically negative. It is concluded that indirect hemagglutination is useful in experimental work and also in estimating the incidence of infection with mycoplasmas in bulls from artificial breeding stations. For diagnostic purposes, use of the indirect hemagglutination test is largely restricted to young bulls and on condition that the first blood sample is drawn very early in the course of the disease.
开展了一项调查，以确定血清学在何种程度上可提供一种手段，用以阐明牛生殖道疾病公牛中牛生殖支原体存在的可能病因学意义。通过间接血凝试验测定，实验感染的公牛早在接种后12天就出现了显著的血清学反应，抗体滴度达到最高。即便经过改进，四氮唑还原抑制试验仍不太适用，因为该方法并未在所有接种动物中检测到抗体。间接血凝试验显示，丹麦和卢森堡种公牛站的大多数公牛血清中抗体滴度很高，不过年轻公牛往往血清学呈阴性。得出的结论是，间接血凝试验在实验工作以及评估人工授精站公牛支原体感染发生率方面都很有用。出于诊断目的，间接血凝试验的使用在很大程度上限于年轻公牛，且条件是在疾病病程极早期采集第一份血样。
