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Indirect hemagglutination with Mycoplasma antigens: effects of pH on antigen sensitization of tanned fresh and formalinized sheep erythrocytes.支原体抗原间接血凝反应：pH值对鞣酸处理的新鲜及甲醛固定绵羊红细胞抗原致敏的影响
Appl Microbiol. 1971 Nov;22(5):756-9. doi: 10.1128/am.22.5.756-759.1971.
2
An indirect haemagglutination test for serum antibodies against mycoplasma pneumoniae using formalinized, tanned sheep erythrocytes.一种使用甲醛鞣制的绵羊红细胞检测血清中抗肺炎支原体抗体的间接血凝试验。
Acta Pathol Microbiol Scand. 1968;73(3):459-72. doi: 10.1111/j.1699-0463.1968.tb04615.x.
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Use of formalinized Mycoplasma gallisepticum antigens and chicken erythrocytes in hemagglutination and hemagglutination-inhibition studies.在血凝和血凝抑制研究中使用福尔马林固定的鸡毒支原体抗原和鸡红细胞。
Appl Microbiol. 1967 Mar;15(2):245-8. doi: 10.1128/am.15.2.245-248.1967.
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[Indirect hemagglutination after fixation in formaldehyde and loading of erythrocytes with tetanus toxoid: method, standardization and indications].
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10
Mycoplasmas in human pyelonephritis: demonstration of antibodies in serum and urine.人类肾盂肾炎中的支原体：血清和尿液中抗体的检测
J Clin Microbiol. 1978 Aug;8(2):197-202. doi: 10.1128/jcm.8.2.197-202.1978.

本文引用的文献

1
SIGNIFICANCE OF ANTIBODY TO MYCOPLASMA HOMINIS TYPE 1 AS MEASURED BY INDIRECT HEMAGGLUTINATION.通过间接血凝法检测人型支原体1型抗体的意义
Proc Soc Exp Biol Med. 1965 Apr;118:1073-83. doi: 10.3181/00379727-118-30049.
2
AN INDIRECT HEMAGGLUTINATION TEST FOR DIAGNOSIS OF MYCOPLASMA PNEUMONIAE INFECTIONS.一种用于诊断肺炎支原体感染的间接血凝试验。
Proc Soc Exp Biol Med. 1964 Aug-Sep;116:947-50. doi: 10.3181/00379727-116-29416.
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HEMAGGLUTINATION STUDIES WITH FORMALINIZED ERYTHROCYTES. EFFECT OF BIS-DIAZO-BENZIDINE AND TANNIC ACID TREATMENT ON SENSITIZATION BY SOLUBLE ANTIGEN.用甲醛处理红细胞的血凝试验。双偶氮联苯胺和鞣酸处理对可溶性抗原致敏作用的影响。
J Immunol. 1963 May;90:663-71.
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Comparison of isolates of Mycoplasma hyorhinis by indirect hemagglutination.
Am J Vet Res. 1963 May;24:622-7.
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Micromethods for the study of proteins and antibodies. I. Procedure and general applications of hemagglutination and hemagglutination-inhibition reactions with tannic acid and protein-treated red blood cells.蛋白质和抗体研究的微量方法。I. 用鞣酸和蛋白质处理的红细胞进行血凝和血凝抑制反应的程序及一般应用。
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6
A study of factors influencing the sensitivity to antibody of tanned, ovalbumin-coated, formalinized sheep red cells.一项关于影响鞣酸处理的、卵白蛋白包被的、甲醛固定的绵羊红细胞抗体敏感性因素的研究。
Immunology. 1967 Nov;13(5):459-68.
7
Use of formalinized Mycoplasma gallisepticum antigens and chicken erythrocytes in hemagglutination and hemagglutination-inhibition studies.在血凝和血凝抑制研究中使用福尔马林固定的鸡毒支原体抗原和鸡红细胞。
Appl Microbiol. 1967 Mar;15(2):245-8. doi: 10.1128/am.15.2.245-248.1967.
8
Effect of pH on the immunogenicity of Mycoplasma pneumoniae.
J Bacteriol. 1969 Feb;97(2):612-9. doi: 10.1128/jb.97.2.612-619.1969.
9
An indirect haemagglutination test for serum antibodies against mycoplasma pneumoniae using formalinized, tanned sheep erythrocytes.一种使用甲醛鞣制的绵羊红细胞检测血清中抗肺炎支原体抗体的间接血凝试验。
Acta Pathol Microbiol Scand. 1968;73(3):459-72. doi: 10.1111/j.1699-0463.1968.tb04615.x.

支原体抗原间接血凝反应：pH值对鞣酸处理的新鲜及甲醛固定绵羊红细胞抗原致敏的影响

Indirect hemagglutination with Mycoplasma antigens: effects of pH on antigen sensitization of tanned fresh and formalinized sheep erythrocytes.

作者信息

Krogsgaard-Jensen A

出版信息

Appl Microbiol. 1971 Nov;22(5):756-9. doi: 10.1128/am.22.5.756-759.1971.

DOI:10.1128/am.22.5.756-759.1971
PMID:5167212
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC376412/
Abstract

An investigation of the influence of different factors affecting the sensitivity of the indirect hemagglutination test has been performed with antigens of four mycoplasmas isolated from sheep or goat. Tanned erythrocytes of sheep, fresh and formalinized, were sensitized with the above antigens. It was demonstrated that, with formalinized erythrocytes, the sensitivity was increased by 50 to 100 times when the sensitization was done at a low pH level. The pH level was unimportant for sensitizing fresh erythrocytes. The greatest sensitivity of the indirect hemagglutination test was obtained with fresh rather than formalinized erythrocytes. Three different types of antigens were used, and the most suitable antigen was found to be the supernatant fluid from an ultrasonically treated centrifuged Mycoplasma suspension.

摘要

利用从绵羊或山羊身上分离出的四种支原体抗原，对影响间接血凝试验敏感性的不同因素进行了研究。用上述抗原致敏新鲜的和经福尔马林固定的绵羊鞣酸红细胞。结果表明，对于经福尔马林固定的红细胞，在低pH值水平下进行致敏时，敏感性提高了50至100倍。pH值水平对新鲜红细胞的致敏并不重要。间接血凝试验用新鲜红细胞而非经福尔马林固定的红细胞可获得最高敏感性。使用了三种不同类型的抗原，发现最合适的抗原是经超声处理的支原体悬液离心后的上清液。