支原体抗原间接血凝反应：pH值对鞣酸处理的新鲜及甲醛固定绵羊红细胞抗原致敏的影响
Indirect hemagglutination with Mycoplasma antigens: effects of pH on antigen sensitization of tanned fresh and formalinized sheep erythrocytes.
作者信息
Krogsgaard-Jensen A
出版信息
Appl Microbiol. 1971 Nov;22(5):756-9. doi: 10.1128/am.22.5.756-759.1971.
Abstract
An investigation of the influence of different factors affecting the sensitivity of the indirect hemagglutination test has been performed with antigens of four mycoplasmas isolated from sheep or goat. Tanned erythrocytes of sheep, fresh and formalinized, were sensitized with the above antigens. It was demonstrated that, with formalinized erythrocytes, the sensitivity was increased by 50 to 100 times when the sensitization was done at a low pH level. The pH level was unimportant for sensitizing fresh erythrocytes. The greatest sensitivity of the indirect hemagglutination test was obtained with fresh rather than formalinized erythrocytes. Three different types of antigens were used, and the most suitable antigen was found to be the supernatant fluid from an ultrasonically treated centrifuged Mycoplasma suspension.
摘要
利用从绵羊或山羊身上分离出的四种支原体抗原，对影响间接血凝试验敏感性的不同因素进行了研究。用上述抗原致敏新鲜的和经福尔马林固定的绵羊鞣酸红细胞。结果表明，对于经福尔马林固定的红细胞，在低pH值水平下进行致敏时，敏感性提高了50至100倍。pH值水平对新鲜红细胞的致敏并不重要。间接血凝试验用新鲜红细胞而非经福尔马林固定的红细胞可获得最高敏感性。使用了三种不同类型的抗原，发现最合适的抗原是经超声处理的支原体悬液离心后的上清液。
相似文献
1
Indirect hemagglutination with Mycoplasma antigens: effects of pH on antigen sensitization of tanned fresh and formalinized sheep erythrocytes.支原体抗原间接血凝反应：pH值对鞣酸处理的新鲜及甲醛固定绵羊红细胞抗原致敏的影响
Appl Microbiol. 1971 Nov;22(5):756-9. doi: 10.1128/am.22.5.756-759.1971.
2
An indirect haemagglutination test for serum antibodies against mycoplasma pneumoniae using formalinized, tanned sheep erythrocytes.一种使用甲醛鞣制的绵羊红细胞检测血清中抗肺炎支原体抗体的间接血凝试验。
Acta Pathol Microbiol Scand. 1968;73(3):459-72. doi: 10.1111/j.1699-0463.1968.tb04615.x.
3
4
Use of formalinized Mycoplasma gallisepticum antigens and chicken erythrocytes in hemagglutination and hemagglutination-inhibition studies.在血凝和血凝抑制研究中使用福尔马林固定的鸡毒支原体抗原和鸡红细胞。
Appl Microbiol. 1967 Mar;15(2):245-8. doi: 10.1128/am.15.2.245-248.1967.
5
An indirect haemagglutination test for detection of antibodies against Mycoplasma hyopneumoniae using formalinized tanned swine erythrocytes.一种使用甲醛鞣制猪红细胞检测猪肺炎支原体抗体的间接血凝试验。
Acta Vet Scand. 1973;14(2):353-5. doi: 10.1186/BF03547458.
6
Differences between Brucella antigens involved in indirect hemagglutination tests with normal and tanned red blood cells.参与正常红细胞和鞣酸处理红细胞间接血凝试验的布鲁氏菌抗原之间的差异。
J Bacteriol. 1967 Sep;94(3):499-505. doi: 10.1128/jb.94.3.499-505.1967.
7
Stable mycoplasma antigen preparations for indirect hemagglutination tests.用于间接血凝试验的稳定支原体抗原制剂。
Appl Microbiol. 1974 Feb;27(2):356-9. doi: 10.1128/am.27.2.356-359.1974.
8
Stable reagent for the detection of antibody to the specific fraction I antigen of Yersinia pestis.用于检测鼠疫耶尔森氏菌特异性I抗原抗体的稳定试剂。
Appl Microbiol. 1972 Apr;23(4):721-4. doi: 10.1128/am.23.4.721-724.1972.
9
Antigenicity of Mycoplasmatales.支原体目抗原性
J Infect Dis. 1973 Mar;127:Suppl:S35-7. doi: 10.1093/infdis/127.supplement_1.s35.
10
[Indirect hemagglutination after fixation in formaldehyde and loading of erythrocytes with tetanus toxoid: method, standardization and indications].
Z Immunitatsforsch Allerg Klin Immunol. 1969 Jun;138(2):178-90.
引用本文的文献
1
Mycoplasma agalactiae, an Etiological Agent of Contagious Agalactia in Small Ruminants: A Review.无乳支原体，小反刍动物传染性无乳症的病原体：综述
Vet Med Int. 2014;2014:286752. doi: 10.1155/2014/286752. Epub 2014 Jul 3.
2
Electrophoretic Analysis of Indian Isolates of Mycoplasma agalactiae and Mycoplasma bovis by SDS-PAGE and Immunoblotting.通过SDS-PAGE和免疫印迹法对无乳支原体和牛支原体印度分离株进行电泳分析
Vet Med Int. 2014;2014:892421. doi: 10.1155/2014/892421. Epub 2014 Apr 3.
3
Antibodies to Chlamydia trachomatis, Mycoplasma hominis, and Neisseria gonorrhoeae in sera from patients with acute salpingitis.急性输卵管炎患者血清中沙眼衣原体、人型支原体和淋病奈瑟菌抗体
Br J Vener Dis. 1981 Apr;57(2):125-9. doi: 10.1136/sti.57.2.125.
4
Mycoplasmal PID: a review of natural and experimental infections.支原体性盆腔炎：自然感染与实验性感染综述
Yale J Biol Med. 1983 Sep-Dec;56(5-6):529-36.
5
Mycoplasmosis: serology of infections in the genital tract of bulls.支原体病：公牛生殖道感染的血清学研究
Infect Immun. 1972 Jan;5(1):20-3. doi: 10.1128/iai.5.1.20-23.1972.
6
Mycoplasmosis: experimental and spontaneous infections of the genital tract of bulls.支原体病：公牛生殖道的实验性感染和自然感染
Acta Vet Scand. 1972;13(2):161-74. doi: 10.1186/BF03548569.
7
Mycoplasma: growth precipitation as a serodiagnostic method.支原体：作为血清诊断方法的生长沉淀法
Appl Microbiol. 1972 Mar;23(3):553-8. doi: 10.1128/am.23.3.553-558.1972.
8
Evaluation of some serological techniques for the identification of Mycoplasma hyorhinis and Mycoplasma suipneumoniae.用于鉴定猪鼻支原体和猪肺炎支原体的一些血清学技术的评估
Acta Vet Scand. 1974;15(2):274-82. doi: 10.1186/BF03547488.
9
Localized immunity in experimental bovine mastitis caused by Mycoplasma dispar.由副支原体引起的实验性牛乳腺炎中的局部免疫
Infect Immun. 1975 Nov;12(5):947-50. doi: 10.1128/iai.12.5.947-950.1975.
10
Mycoplasmas in human pyelonephritis: demonstration of antibodies in serum and urine.人类肾盂肾炎中的支原体：血清和尿液中抗体的检测
J Clin Microbiol. 1978 Aug;8(2):197-202. doi: 10.1128/jcm.8.2.197-202.1978.
本文引用的文献
1
SIGNIFICANCE OF ANTIBODY TO MYCOPLASMA HOMINIS TYPE 1 AS MEASURED BY INDIRECT HEMAGGLUTINATION.通过间接血凝法检测人型支原体1型抗体的意义
Proc Soc Exp Biol Med. 1965 Apr;118:1073-83. doi: 10.3181/00379727-118-30049.
2
AN INDIRECT HEMAGGLUTINATION TEST FOR DIAGNOSIS OF MYCOPLASMA PNEUMONIAE INFECTIONS.一种用于诊断肺炎支原体感染的间接血凝试验。
Proc Soc Exp Biol Med. 1964 Aug-Sep;116:947-50. doi: 10.3181/00379727-116-29416.
3
4
Comparison of isolates of Mycoplasma hyorhinis by indirect hemagglutination.
Am J Vet Res. 1963 May;24:622-7.
5
6
7
Use of formalinized Mycoplasma gallisepticum antigens and chicken erythrocytes in hemagglutination and hemagglutination-inhibition studies.在血凝和血凝抑制研究中使用福尔马林固定的鸡毒支原体抗原和鸡红细胞。
Appl Microbiol. 1967 Mar;15(2):245-8. doi: 10.1128/am.15.2.245-248.1967.
8
Effect of pH on the immunogenicity of Mycoplasma pneumoniae.
J Bacteriol. 1969 Feb;97(2):612-9. doi: 10.1128/jb.97.2.612-619.1969.
9
An indirect haemagglutination test for serum antibodies against mycoplasma pneumoniae using formalinized, tanned sheep erythrocytes.一种使用甲醛鞣制的绵羊红细胞检测血清中抗肺炎支原体抗体的间接血凝试验。
Acta Pathol Microbiol Scand. 1968;73(3):459-72. doi: 10.1111/j.1699-0463.1968.tb04615.x.
Zotero 插件