Chun P W, Yang M C
Biophys Chem. 1979 May;9(4):313-28.
Large zone reaction boundary profiles for molecular sieve chromatography as affected by kinetic parameters have been simulated for local equilibration between the mobile and stationary phases. Our studies of monomer-dimer and monomer-tetramer systems indicate that in a slowly equilibrating system, the kinetic controls operating between the mobile and stationary phases contribute most significantly to the overall boundary profile. In a rapidly equilibrating system, however, the kinetic parameters kij and kji operating in the mobile phase are the principal determinants of the reaction boundary, while the kinetic effects of kii and k-ii between the mobile and stationary phases are minimal.
针对流动相和固定相之间的局部平衡，模拟了受动力学参数影响的分子筛色谱大区域反应边界轮廓。我们对单体 - 二聚体和单体 - 四聚体系统的研究表明，在缓慢平衡的系统中，流动相和固定相之间运行的动力学控制对整体边界轮廓的贡献最为显著。然而，在快速平衡的系统中，流动相中运行的动力学参数kij和kji是反应边界的主要决定因素，而流动相和固定相之间的kii和k-ii的动力学效应则最小。
