豚鼠大脑皮层中的胆碱代谢。磷酸胆碱和脂质胆碱。
Choline metabolism in the cerebral cortex of guinea pigs. Phosphorylcholine and lipid choline.
作者信息
Dowdall M J, Barker L A, Whittaker V P
出版信息
Biochem J. 1972 Dec;130(4):1081-94. doi: 10.1042/bj1301081.
Abstract
- The labelling of phosphorylcholine and choline-containing phospholipids in the subcellular fractions of guinea-pig cerebral cortex after the intraventricular injection of [N-Me-(3)H]choline into conscious animals has been studied. Special emphasis was placed upon the synaptosome fraction and early time-periods after administration. 2. The labelling of phosphorylcholine was rapid compared with that of phospholipid and was confined to two distinct subcellular fractions: the soluble cytoplasmic fraction and the synaptosome fraction. Most of the labelled phosphorylcholine of the synaptosome fraction was readily released by osmotic rupture indicating location in the nerve-ending cytoplasm. The two pools of phosphorylcholine had similar specific radioactivities at all observed times. 3. (3)H-labelled phospholipid was found in all membranous fractions. The labelling was confined to choline-containing phospholipids, notably phosphatidylcholine. 4. The labelling of the different membranous fractions was similar. 5. The half-life of the choline-containing phospholipids in the synaptic vesicle fraction was very much greater than the acetylcholine in this fraction. 6. Evidence is presented that synthesis de novo of phosphatidylcholine at nerve terminals occurs in vivo.
摘要
- 研究了向清醒动物脑室内注射[N-甲基-(3)H]胆碱后，豚鼠大脑皮质亚细胞组分中磷酸胆碱和含胆碱磷脂的标记情况。特别关注了突触体组分以及给药后的早期时间段。2. 与磷脂相比，磷酸胆碱的标记速度较快，且局限于两个不同的亚细胞组分：可溶性细胞质组分和突触体组分。突触体组分中大部分标记的磷酸胆碱可通过渗透破裂轻易释放，表明其位于神经末梢细胞质中。在所有观察时间点，这两个磷酸胆碱池具有相似的比放射性。3. 在所有膜性组分中均发现了(3)H标记的磷脂。标记局限于含胆碱的磷脂，尤其是磷脂酰胆碱。4. 不同膜性组分的标记情况相似。5. 突触小泡组分中含胆碱磷脂的半衰期比该组分中的乙酰胆碱长得多。6. 有证据表明，神经末梢处磷脂酰胆碱的从头合成在体内发生。
相似文献
1
Choline metabolism in the cerebral cortex of guinea pigs. Phosphorylcholine and lipid choline.豚鼠大脑皮层中的胆碱代谢。磷酸胆碱和脂质胆碱。
Biochem J. 1972 Dec;130(4):1081-94. doi: 10.1042/bj1301081.
2
Choline metabolism in the cerebral cortex of guinea pigs. Stable-bound acetylcholine.豚鼠大脑皮层中的胆碱代谢。稳定结合的乙酰胆碱。
Biochem J. 1972 Dec;130(4):1063-75. doi: 10.1042/bj1301063.
3
Isolation of ( 3 H) acetylcholine pools by subcellular fractionation of cerebral cortex slices incubated with ( 3 H) choline.通过对用（³H）胆碱孵育的大脑皮层切片进行亚细胞分级分离来分离（³H）乙酰胆碱池。
J Neurochem. 1971 May;18(5):705-12. doi: 10.1111/j.1471-4159.1971.tb12000.x.
4
The incorporation of radioactive fatty acids and of radioactive derivatives of glucose into the phospholipids of subsynaptosomal fractions of cerebral cortex.放射性脂肪酸和葡萄糖的放射性衍生物掺入大脑皮质突触下组分的磷脂中。
Biochem J. 1976 Jan 15;154(1):65-75. doi: 10.1042/bj1540065.
5
The involvement of lysophosphoglycerides in neurotransmitter release; the composition and turnover of phospholipids of synaptic vesicles of guinea-pig cerebral cortex and Torpedo electric organ and the effect of stimulation.溶血磷酸甘油酯在神经递质释放中的作用；豚鼠大脑皮层和电鳐电器官突触小泡磷脂的组成与更新以及刺激的影响。
Brain Res. 1975 Dec 26;100(3):629-44. doi: 10.1016/0006-8993(75)90162-6.
6
Incorporation of (Me-14C)choline into phosphatidyl choline of rat cerebral cortex membranes in vitro.体外将（甲基 - 14C）胆碱掺入大鼠大脑皮质膜的磷脂酰胆碱中。
Brain Res. 1970 Mar 17;18(3):451-9. doi: 10.1016/0006-8993(70)90128-9.
7
Studies on the turnover of endogenous choline-containing phospholipids of cultured neuroblastoma cells.培养的神经母细胞瘤细胞内源性含胆碱磷脂周转的研究。
Biochim Biophys Acta. 1983 Aug 1;752(3):467-73. doi: 10.1016/0005-2760(83)90277-1.
8
Can mitochondria and synaptosomes of guinea-pig brain synthesize phospholipids?豚鼠脑的线粒体和突触体能够合成磷脂吗？
Biochem J. 1972 Feb;126(4):805-21. doi: 10.1042/bj1260805.
9
The turnover of phosphatidyl choline in rat cerebral cortex membranes in vivo.大鼠大脑皮质膜中磷脂酰胆碱在体内的周转情况。
J Neurobiol. 1969;1(3):295-302. doi: 10.1002/neu.480010305.
10
The incorporation of triethylcholine into isolated guinea pig cerebral cortex synaptosoma and synaptic vesicle fractions.将三乙胆碱掺入分离的豚鼠大脑皮质突触体和突触小泡组分中。
Eur J Pharmacol. 1971 Oct;16(2):164-70. doi: 10.1016/0014-2999(71)90006-9.
引用本文的文献
1
Multiple activities of sphingomyelin synthase 2 generate saturated fatty acid- and/or monounsaturated fatty acid-containing diacylglycerol.鞘磷脂合酶2的多种活性产生含饱和脂肪酸和/或单不饱和脂肪酸的二酰基甘油。
J Biol Chem. 2024 Dec;300(12):107960. doi: 10.1016/j.jbc.2024.107960. Epub 2024 Nov 5.
2
A proton spectroscopy study of white matter in children with autism.一项针对自闭症儿童白质的质子光谱研究。
Prog Neuropsychopharmacol Biol Psychiatry. 2016 Apr 3;66:48-53. doi: 10.1016/j.pnpbp.2015.11.005. Epub 2015 Nov 16.
3
Choline metabolism in the cerebral cortex of guinea pigs. Stable-bound acetylcholine.豚鼠大脑皮层中的胆碱代谢。稳定结合的乙酰胆碱。
Biochem J. 1972 Dec;130(4):1063-75. doi: 10.1042/bj1301063.
4
Uptake and metabolism of [3H]choline by the rat phrenic nerve-hemidiaphragm preparation.大鼠膈神经-半横膈标本对[3H]胆碱的摄取与代谢
Naunyn Schmiedebergs Arch Pharmacol. 1987 Mar;335(3):231-7. doi: 10.1007/BF00172789.
5
Analysis of the water-soluble products of phosphatidylcholine breakdown by ion-exchange chromatography. Bombesin and TPA (12-O-tetradecanoylphorbol 13-acetate) stimulate choline generation in Swiss 3T3 cells by a common mechanism.通过离子交换色谱法分析磷脂酰胆碱分解的水溶性产物。蛙皮素和TPA（12-O-十四烷酰佛波醇13-乙酸酯）通过共同机制刺激瑞士3T3细胞中胆碱的生成。
Biochem J. 1989 Oct 15;263(2):581-7. doi: 10.1042/bj2630581.
6
The fate of choline in the circulating plasma of the rat.大鼠循环血浆中胆碱的命运
Experientia. 1979 Feb 15;35(2):211-2. doi: 10.1007/BF01920622.
7
Choline kinase and ethanolamine kinase activity in the cytosol of nerve endings from rat forebrain.大鼠前脑神经末梢胞质溶胶中的胆碱激酶和乙醇胺激酶活性。
Biochem J. 1979 Mar 15;178(3):753-60. doi: 10.1042/bj1780753.
本文引用的文献
1
An improved method for the colorimetric determination of phosphate.一种改进的比色法测定磷酸盐的方法。
Biochem J. 1938 Feb;32(2):295-8. doi: 10.1042/bj0320295.
2
Separation of the phosphoric esters on the filter paper chromatogram.磷酸酯在滤纸色谱上的分离
Nature. 1949 Dec 31;164(4183):1107-12, illust. doi: 10.1038/1641107a0.
3
FORM-CELL RETICULOSIS OF MICE: AN INHERITED CONDITION RESEMBLING GAUCHER'S AND NIERMANN-PICK DISEASES.小鼠的泡沫细胞网状细胞增多症：一种类似高雪氏病和尼曼-皮克病的遗传性疾病。
J Med Genet. 1965 Jun;2(2):99-106. doi: 10.1136/jmg.2.2.99.
4
A hydrolytic procedure for the identification and estimation of individual phospholipids in biological samples.一种用于鉴定和估算生物样品中单个磷脂的水解方法。
Biochem J. 1960 Apr;75(1):45-53. doi: 10.1042/bj0750045.
5
An improved method for the determination of free and total cholesterol using the ferric chloride reaction.一种使用氯化铁反应测定游离胆固醇和总胆固醇的改进方法。
Clin Chim Acta. 1958 Jul;3(4):357-67. doi: 10.1016/0009-8981(58)90025-1.
6
A simple method for the isolation and purification of total lipides from animal tissues.一种从动物组织中分离和纯化总脂质的简单方法。
J Biol Chem. 1957 May;226(1):497-509.
7
The function of cytidine coenzymes in the biosynthesis of phospholipides.胞苷辅酶在磷脂生物合成中的作用。
J Biol Chem. 1956 Sep;222(1):193-214.
8
[Lecithinase C in animal tissue].[动物组织中的卵磷脂酶C]
Biokhimiia. 1952 Jan-Feb;17(1):77-81.
9
Distribution of lipids in subcellular particles of guinea-pig brain.豚鼠脑亚细胞颗粒中脂质的分布
Biochem J. 1964 Jul;92(1):91-100. doi: 10.1042/bj0920091.
10
The separation of synaptic vesicles from nerve-ending particles ('synaptosomes').突触小泡与神经末梢颗粒（“突触体”）的分离。
Biochem J. 1964 Feb;90(2):293-303. doi: 10.1042/bj0900293.
Zotero 插件