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1
Analysis of the water-soluble products of phosphatidylcholine breakdown by ion-exchange chromatography. Bombesin and TPA (12-O-tetradecanoylphorbol 13-acetate) stimulate choline generation in Swiss 3T3 cells by a common mechanism.通过离子交换色谱法分析磷脂酰胆碱分解的水溶性产物。蛙皮素和TPA（12-O-十四烷酰佛波醇13-乙酸酯）通过共同机制刺激瑞士3T3细胞中胆碱的生成。
Biochem J. 1989 Oct 15;263(2):581-7. doi: 10.1042/bj2630581.
2
Hydrolysis of phosphatidylcholine by phospholipase D is a common response to mitogens which stimulate inositol lipid hydrolysis in Swiss 3T3 fibroblasts.
Biochim Biophys Acta. 1991 Apr 17;1092(2):265-72. doi: 10.1016/0167-4889(91)90166-u.
3
A tumour promoter, 12-O-tetradecanoylphorbol 13-acetate, increases cellular 1,2-diacylglycerol content through a mechanism other than phosphoinositide hydrolysis in Swiss-mouse 3T3 fibroblasts.一种肿瘤促进剂，12 - O - 十四酰佛波醇13 - 乙酸酯，通过一种不同于瑞士小鼠3T3成纤维细胞中磷酸肌醇水解的机制增加细胞1,2 - 二酰甘油含量。
Biochem J. 1987 May 1;243(3):647-53. doi: 10.1042/bj2430647.
4
Bombesin and phorbol ester stimulate phosphatidylcholine hydrolysis by phospholipase C: evidence for a role of protein kinase C.蛙皮素和佛波酯通过磷脂酶C刺激磷脂酰胆碱水解：蛋白激酶C作用的证据。
J Cell Physiol. 1987 Mar;130(3):382-91. doi: 10.1002/jcp.1041300311.
5
Stimulation of phosphatidylcholine breakdown and diacylglycerol production by growth factors in Swiss-3T3 cells.生长因子对瑞士3T3细胞中磷脂酰胆碱分解和二酰基甘油生成的刺激作用。
Biochem J. 1989 Dec 1;264(2):509-15. doi: 10.1042/bj2640509.
6
The regulation of phospholipase D activity and its role in sn-1,2-diradylglycerol formation in bombesin- and phorbol 12-myristate 13-acetate-stimulated Swiss 3T3 cells.蛙皮素和佛波酯刺激的瑞士3T3细胞中磷脂酶D活性的调节及其在sn-1,2-二酰甘油形成中的作用
Biochem J. 1991 Dec 1;280 ( Pt 2)(Pt 2):431-8. doi: 10.1042/bj2800431.
7
Epidermal growth factor increases sn-1,2-diacylglycerol levels and activates phospholipase D-catalysed phosphatidylcholine breakdown in Swiss 3T3 cells in the absence of inositol-lipid hydrolysis.在不发生肌醇脂质水解的情况下，表皮生长因子可提高瑞士3T3细胞中sn-1,2-二酰甘油水平，并激活磷脂酶D催化的磷脂酰胆碱分解。
Biochem J. 1992 Jul 1;285 ( Pt 1)(Pt 1):247-53. doi: 10.1042/bj2850247.
8
Phosphatidylcholine metabolism in nuclei of phorbol ester-activated LA-N-1 neuroblastoma cells.佛波酯激活的LA-N-1神经母细胞瘤细胞核中的磷脂酰胆碱代谢
Neurochem Res. 2000 Aug;25(8):1073-82. doi: 10.1023/a:1007613827552.
9
Stimulation of the hydrolysis of phosphatidylinositol 4,5-bisphosphate and phosphatidylcholine by endothelin, a complete mitogen for Rat-1 fibroblasts.内皮素（一种对大鼠-1成纤维细胞有完全促有丝分裂作用的物质）对磷脂酰肌醇4,5-二磷酸和磷脂酰胆碱水解的刺激作用。
Biochem J. 1990 Dec 15;272(3):761-6. doi: 10.1042/bj2720761.
10
Choline derived from the phosphatidylcholine specific phospholipase D is not directly available for the CDP choline pathway in phorbol ester-treated C3H10T1/2 Cl 8 fibroblasts.在佛波酯处理的C3H10T1/2 Cl 8成纤维细胞中，源自磷脂酰胆碱特异性磷脂酶D的胆碱不能直接用于CDP胆碱途径。
Mol Cell Biochem. 1998 Oct;187(1-2):147-54. doi: 10.1023/a:1006813524791.

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1
Loss of Ypk1, the yeast homolog to the human serum- and glucocorticoid-induced protein kinase, accelerates phospholipase B1-mediated phosphatidylcholine deacylation.失活酵母中与人血清和糖皮质激素诱导的蛋白激酶同源的 Ypk1，可加速磷脂酶 B1 介导的卵磷脂脱酰基作用。
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2
Effects of flutamide on [methyl-(3)h]-choline uptake in human prostate cancer-3 cells: a pilot study.氟他胺对人前列腺癌-3细胞中[甲基-(3)H]-胆碱摄取的影响：一项初步研究。
Curr Ther Res Clin Exp. 2007 Jul;68(4):226-41. doi: 10.1016/j.curtheres.2007.08.003.
3
Glycerophosphocholine utilization by Candida albicans: role of the Git3 transporter in virulence.白色念珠菌对甘油磷酸胆碱的利用：Git3 转运蛋白在毒力中的作用。
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4
Human prostate cell lines from normal and tumourigenic epithelia differ in the pattern and control of choline lipid headgroups released into the medium on stimulation of protein kinase C.在蛋白激酶 C 刺激下，正常和致瘤上皮来源的人前列腺细胞系在释放到培养基中的胆碱脂质头部的模式和控制上存在差异。
Br J Cancer. 2011 Feb 15;104(4):673-84. doi: 10.1038/sj.bjc.6606077. Epub 2011 Jan 25.
5
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6
Inhibition of Plasmodium falciparum choline kinase by hexadecyltrimethylammonium bromide: a possible antimalarial mechanism.十六烷基三甲基溴化铵对恶性疟原虫胆碱激酶的抑制作用：一种可能的抗疟机制。
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7
Regulation of activity in vitro and in vivo of three phospholipases B from Saccharomyces cerevisiae.酿酒酵母中三种磷脂酶B在体外和体内的活性调节
Biochem J. 2005 Apr 15;387(Pt 2):489-96. doi: 10.1042/BJ20041272.
8
Phosphatidylcholine metabolism in nuclei of phorbol ester-activated LA-N-1 neuroblastoma cells.佛波酯激活的LA-N-1神经母细胞瘤细胞核中的磷脂酰胆碱代谢
Neurochem Res. 2000 Aug;25(8):1073-82. doi: 10.1023/a:1007613827552.
9
Ionotrophic 5-hydroxytryptamine type 3 receptor activates the protein kinase C-dependent phospholipase D pathway in human T-cells.离子型5-羟色胺3型受体激活人T细胞中蛋白激酶C依赖性磷脂酶D途径。
Biochem J. 1999 Nov 15;344 Pt 1(Pt 1):199-204.
10
Characterization of the regulation of phospholipase D activity in the detergent-insoluble fraction of HL60 cells by protein kinase C and small G-proteins.蛋白激酶C和小G蛋白对HL60细胞去污剂不溶性组分中磷脂酶D活性调节的表征
Biochem J. 1999 Apr 1;339 ( Pt 1)(Pt 1):87-93.

本文引用的文献

1
Alkaline O leads to N-transacylation. A new method for the quantitative deacylation of phospholipids.碱性O导致N-转酰基化。一种磷脂定量脱酰基化的新方法。
Biochem J. 1981 Apr 1;195(1):301-6. doi: 10.1042/bj1950301.
2
Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters.肿瘤促进剂佛波酯对钙激活的、磷脂依赖性蛋白激酶的直接激活作用。
J Biol Chem. 1982 Jul 10;257(13):7847-51.
3
Regulation of phosphatidylcholine biosynthesis by mitogenic growth factors.有丝分裂原性生长因子对磷脂酰胆碱生物合成的调控
Biochim Biophys Acta. 1984 Mar 7;792(3):270-80. doi: 10.1016/0005-2760(84)90194-2.
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Is phosphatidic acid a calcium ionophore under neurohumoral control?在神经体液控制下，磷脂酸是一种钙离子载体吗？
Nature. 1980 Mar 27;284(5754):345-7. doi: 10.1038/284345a0.
5
Disappearance of Ca2+-sensitive, phospholipid-dependent protein kinase activity in phorbol ester-treated 3T3 cells.佛波酯处理的3T3细胞中钙敏感、磷脂依赖性蛋白激酶活性的消失。
Biochem Biophys Res Commun. 1984 May 16;120(3):1053-9. doi: 10.1016/s0006-291x(84)80213-2.
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Reduction of epidermal growth factor receptor affinity by heterologous ligands: evidence for a mechanism involving the breakdown of phosphoinositides and the activation of protein kinase C.异源配体降低表皮生长因子受体亲和力：涉及磷酸肌醇分解和蛋白激酶C激活机制的证据
Biochem Biophys Res Commun. 1984 Aug 30;123(1):377-84. doi: 10.1016/0006-291x(84)90424-8.
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Choline metabolism in the cerebral cortex of guinea pigs. Phosphorylcholine and lipid choline.豚鼠大脑皮层中的胆碱代谢。磷酸胆碱和脂质胆碱。
Biochem J. 1972 Dec;130(4):1081-94. doi: 10.1042/bj1301081.
8
Quantitative analysis of phospholipids by thin-layer chromatography.通过薄层色谱法对磷脂进行定量分析。
Biochem J. 1964 Feb;90(2):374-8. doi: 10.1042/bj0900374.
9
Growth factor-like action of phosphatidic acid.磷脂酸的生长因子样作用。
Nature. 1986;323(6084):171-3. doi: 10.1038/323171a0.
10
1,2-Diacylglycerols and phorbol esters stimulate phosphatidylcholine metabolism in GH3 pituitary cells. Evidence for separate mechanisms of action.1,2-二酰基甘油和佛波酯刺激GH3垂体细胞中的磷脂酰胆碱代谢。作用机制不同的证据。
J Biol Chem. 1987 Jul 5;262(19):9204-10.

通过离子交换色谱法分析磷脂酰胆碱分解的水溶性产物。蛙皮素和TPA（12-O-十四烷酰佛波醇13-乙酸酯）通过共同机制刺激瑞士3T3细胞中胆碱的生成。

Analysis of the water-soluble products of phosphatidylcholine breakdown by ion-exchange chromatography. Bombesin and TPA (12-O-tetradecanoylphorbol 13-acetate) stimulate choline generation in Swiss 3T3 cells by a common mechanism.

作者信息

Cook S J, Wakelam M J

机构信息

Department of Biochemistry, University of Glasgow, Scotland, U.K.

出版信息

Biochem J. 1989 Oct 15;263(2):581-7. doi: 10.1042/bj2630581.

DOI:10.1042/bj2630581
PMID:2597122
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1133466/
Abstract

A method for the rapid and quantitative separation of glycerophosphocholine, choline phosphate and choline upon ion-exchange columns is described. The method has been utilized to examine the stimulation of phosphatidylcholine breakdown in quiescent Swiss 3T3 cells in response to bombesin and 12-O-tetradecanoylphorbol 13-acetate (TPA). The stimulated generation of choline is shown to precede that of choline phosphate, with no effect upon glycerophosphocholine levels; but was attenuated in cells in which protein kinase C activity was down-regulated. The results thus suggest that stimulation of the cells with either bombesin or TPA activates phospholipase D-catalysed phosphatidylcholine breakdown by a common mechanism involving the activation of protein kinase C.

摘要

本文描述了一种在离子交换柱上快速定量分离甘油磷酸胆碱、磷酸胆碱和胆碱的方法。该方法已被用于检测在静止的瑞士3T3细胞中，蛙皮素和12-O-十四酰佛波醇-13-乙酸酯（TPA）对磷脂酰胆碱分解的刺激作用。结果表明，胆碱的刺激生成先于磷酸胆碱，对甘油磷酸胆碱水平无影响；但在蛋白激酶C活性下调的细胞中，这种刺激作用减弱。因此，结果表明用蛙皮素或TPA刺激细胞，通过涉及蛋白激酶C激活的共同机制，激活磷脂酶D催化的磷脂酰胆碱分解。