Glasser S R, Chytil F, Spelsberg T C
Biochem J. 1972 Dec;130(4):947-57. doi: 10.1042/bj1300947.
Oestradiol-17beta (1.0mug) was injected intravenously into ovariectomized rats. The earliest detectable hormonal response in isolated uterine nuclei was an increase (10-15min) in RNA polymerase II activity (DNA-like RNA synthesis), which reached a peak at 30min and then decreased to control values (by 1-2h) before displaying a second increase over control activity from 2 to 12h. The next response to oestradiol-17beta was an increase (30-60min) in polymerase I activity (rRNA synthesis) and template capacity of the chromatin. The concentrations of acidic chromatin proteins did not begin to increase until 1h after injection of oestradiol-17beta and histone concentrations showed no significant changes during the 8h period after administration. The early (15min) increase in RNA synthesis in ;high-salt conditions' can be completely eliminated by alpha-amanitin, an inhibitor of the RNA polymerase II. The exact nature of this early increase in endogenous polymerase II activity remains to be determined, e.g. whether it is caused by the increased availability of transcribable DNA of the chromatin or via direct hormonal activation of the enzyme per se.
将17β-雌二醇（1.0微克）静脉注射到去卵巢大鼠体内。在分离的子宫核中最早可检测到的激素反应是RNA聚合酶II活性（DNA样RNA合成）增加（10 - 15分钟），在30分钟时达到峰值，然后在1 - 2小时降至对照值，之后在2至12小时内出现高于对照活性的第二次增加。对17β-雌二醇的下一个反应是聚合酶I活性（rRNA合成）和染色质模板能力增加（30 - 60分钟）。酸性染色质蛋白的浓度直到注射17β-雌二醇1小时后才开始增加，组蛋白浓度在给药后的8小时内没有显著变化。在“高盐条件”下RNA合成的早期（15分钟）增加可被RNA聚合酶II抑制剂α-鹅膏蕈碱完全消除。内源性聚合酶II活性这种早期增加的确切性质仍有待确定，例如，它是由染色质可转录DNA可用性增加引起的，还是通过该酶本身的直接激素激活引起的。
