Sinatra R S, Ford D H, Rhines R K
Brain Res. 1979 Aug 3;171(2):307-17. doi: 10.1016/0006-8993(79)90335-4.
Although opiates significantly alter RNA and protein synthesis in a variety of neuronal cell types, their effect on the biosynthetic activity of regenerating neurons has not been investigated. In the present study, the effect of morphine on the incorporation of [3H]L-lysine into proteins of facial nucleus neurons was examined by light microscopic radioautography. Silver grains present within various compartments of normal and regenerating (3-, 7-, 14- and 21 days post-axotomy) neurons from saline-treated Wistar rats were compared with the amount present in similar cells from animals receiving 40 mg/kg morphine sulfate i.v. At 14- and 21-days post-axotomy, regenerating neurons were larger and the grain count in the emulsion over these cells was greater than that observed in normal (unoperated) neurons. In normal facial neurons, the accumulation of lysine into the nucleus and nucleolus was significantly lower 60 min after morphine administration. However, morphine's inhibition of lysine incorporation was even more pronounced in regenerating neurons. In these cells, nuclear lysine uptake was depressed at 3 and 7 days, while maximum inhibition of cytoplasmic incorporation occurred at 14-days post-axotomy. Morphine administration decreased nucleolar lysine incorporation at all survival intervals.
尽管阿片类药物能显著改变多种神经元细胞类型中的RNA和蛋白质合成，但它们对再生神经元生物合成活性的影响尚未得到研究。在本研究中，通过光学显微镜放射自显影术检测了吗啡对[3H]L-赖氨酸掺入面神经核神经元蛋白质的影响。将生理盐水处理的Wistar大鼠正常和再生（轴突切断后3、7、14和21天）神经元各部分内的银颗粒数量，与静脉注射40mg/kg硫酸吗啡的动物相似细胞中的银颗粒数量进行比较。在轴突切断后14天和21天，再生神经元更大，这些细胞上乳剂中的颗粒计数高于正常（未手术）神经元。在正常面神经神经元中，吗啡给药60分钟后，赖氨酸在细胞核和核仁中的积累显著降低。然而，吗啡对赖氨酸掺入的抑制在再生神经元中更为明显。在这些细胞中，轴突切断后3天和7天，细胞核赖氨酸摄取受到抑制，而细胞质掺入的最大抑制发生在轴突切断后14天。在所有存活时间间隔，吗啡给药均降低了核仁赖氨酸掺入。
