McLoon L K, LaVelle A
Brain Res. 1981 Apr;227(2):237-48. doi: 10.1016/0165-3806(81)90111-5.
Tritiated leucine incorporation was examined after either crush or axotomy of the hamster facial nerve at specific stages in the maturation of the neuronal nucleolus. Changes in the neuronal metabolic response to injury in development were demonstrated with liquid scintillation examination of tritiated leucine incorporation into the trichloroacetic acid (TCA)-insoluble and TCA-soluble fractions derived from whole reactive and normal facial nuclear groups. Changes in incorporation seen in the developmental sequence were attributed to actual changes in neuronal protein metabolism, and not to changes in the amino acid pool, glial changes or hyperemic capillary changes. The ability to increase leucine incorporation over the normal as a result of injury in development coincided with the time of final nucleolar maturation in the facial motor neurons, beginning at approximately 20 days postnatal age. Thus, there is a correlation between a specific morphological event, the attainment of the mature nucleolar configuration, and the acquisition of the mature synthetic capacity as indicated by the ability to respond to injury in the mature manner.
在仓鼠面神经成熟的特定阶段，分别对面神经进行挤压或切断后，检测了氚标记亮氨酸的掺入情况。通过液体闪烁法检测氚标记亮氨酸掺入来自整个反应性和正常面神经核团的三氯乙酸（TCA）不溶性和TCA可溶性部分，证实了发育过程中神经元对损伤的代谢反应变化。发育序列中掺入的变化归因于神经元蛋白质代谢的实际变化，而不是氨基酸池、神经胶质变化或充血性毛细血管变化。发育过程中因损伤而使亮氨酸掺入量超过正常水平的能力，与面部运动神经元核仁最终成熟的时间一致，大约从出生后20天开始。因此，特定的形态学事件（成熟核仁构型的形成）与成熟合成能力的获得（以成熟方式对损伤作出反应的能力所表明）之间存在相关性。
