胆甾-5-烯-3,26-二醇作为肝脏线粒体将胆固醇转化为胆汁酸过程中的中间体的形成。
The formation of cholest-5-ene-3 ,26-diol as an intermediate in the conversion of cholesterol into bile acids by liver mitochondria.
作者信息
Mitropoulos K A, Avery M D, Myant N B, Gibbons G F
出版信息
Biochem J. 1972 Nov;130(2):363-71. doi: 10.1042/bj1300363.
Abstract
- When [(14)C]cholesterol was incubated with rat liver mitochondria, radioactive 26-hydroxycholesterol, 3beta-hydroxychol-5-enoic acid and other bile acids were isolated from the incubation mixture. 2. In the absence of added 26-hydroxycholesterol, the specific radioactivity of the 26-hydroxycholesterol formed from [(14)C]cholesterol during the incubation was higher than that of the 3beta-hydroxychol-5-enoic acid. Addition of increasing amounts of 26-hydroxycholesterol led to a progressive fall in the specific radioactivity, and to a progressive increase in the mass, of the 3beta-hydroxychol-5-enoic acid recovered at the end of the incubation. 3. It is concluded that 26-hydroxycholesterol is an intermediate in the formation of 3beta-hydroxychol-5-enoic acid from cholesterol. 4. Comparison of the specific radioactivity of the 26-hydroxycholesterol formed in the incubation mixture with that of the added [(14)C]cholesterol indicates that endogenous cholesterol in mitochondria is accessible to cholesterol 26-hydroxylase.
摘要
- 当将[¹⁴C]胆固醇与大鼠肝线粒体一起温育时，从温育混合物中分离出了放射性的26-羟胆固醇、3β-羟基胆-5-烯酸和其他胆汁酸。2. 在未添加26-羟胆固醇的情况下，温育过程中由[¹⁴C]胆固醇形成的26-羟胆固醇的比放射性高于3β-羟基胆-5-烯酸的比放射性。添加越来越多的26-羟胆固醇会导致温育结束时回收的3β-羟基胆-5-烯酸的比放射性逐渐下降，而质量逐渐增加。3. 得出的结论是，26-羟胆固醇是胆固醇形成3β-羟基胆-5-烯酸过程中的中间体。4. 将温育混合物中形成的26-羟胆固醇的比放射性与添加的[¹⁴C]胆固醇的比放射性进行比较表明，线粒体中的内源性胆固醇可被胆固醇26-羟化酶作用。
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Biochim Biophys Acta. 1967 Oct 2;144(2):430-9. doi: 10.1016/0005-2760(67)90173-7.
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