乙醇对分离的大鼠肝细胞中氯乙烯代谢的抑制作用。

Ethanol inhibition of vinyl chloride metabolism in isolated rat hepatocytes.

作者信息

Hultmark D, Sundh K, Johansson L, Arrhenius E

出版信息

Chem Biol Interact. 1979 Apr;25(1):1-6. doi: 10.1016/0009-2797(79)90064-4.

Abstract

Isolated rat liver cells convert [14C]vinyl chloride into non-volatile metabolites. The metabolism is not increased by in vivo pretreatment with phenobarbital. It is sensitive to inhibition by ethanol, which at a concentration of 4 mM inhibits vinyl chloride metabolism to 50% in hepatocyte suspensions. The metabolic activity is NADPH-dependent and is localized in the microsomal fraction of the liver. The enzyme is also strongly inhibited by tetrahydrofuran, indicating that it could be identical to an ethanol-inducible cytochrome P-450 described in the literature [1].

摘要

分离的大鼠肝细胞可将[14C]氯乙烯转化为非挥发性代谢产物。苯巴比妥的体内预处理不会增加这种代谢作用。它对乙醇抑制敏感，在肝细胞悬液中，4 mM浓度的乙醇可将氯乙烯代谢抑制50%。这种代谢活性依赖于NADPH，且定位于肝脏的微粒体部分。该酶也受到四氢呋喃的强烈抑制，这表明它可能与文献[1]中描述的乙醇诱导型细胞色素P-450相同。