Scribner N K, Scribner J D
Chem Biol Interact. 1979 Jun;26(1):47-55. doi: 10.1016/0009-2797(79)90092-9.
N-Acetoxy-4-acetamidostilbene (N-AcO-AAS) has been shown to react with mononucleosides to give numerous alkylation products [1]. In this work, homopolynucleotides, RNA and DNA were treated with N-[beta-14 C]-AcO-AAS, washed, degraded with S1 nuclease and acid phosphatase, and chromatographed on Sephadex LH-20. RNA prepared in vitro with 14C on cytosine, adenine or guanine was treated with non-radioactive N-AcO-AAS, then digested and chromatographed similarly. By this means, many of the adducts rising from nucleoside reactions were shown to result from treatment of nucleic acids with the same carcinogen, as well as a number of products which have not been matched to products of monomer alkylation. Labeled 1-(4-acetamidophenyl)-2-phenyl-1, 2-ethanediol was detected in the digest of RNA treated with radioactive N-AcO-AAS, suggesting that phosphate alkylation had taken place.
N-乙酰氧基-4-乙酰氨基芪（N-AcO-AAS）已被证明可与单核苷反应生成多种烷基化产物[1]。在本研究中，将同聚核苷酸、RNA和DNA用N-[β-14C]-AcO-AAS处理，洗涤后，用S1核酸酶和酸性磷酸酶降解，并在葡聚糖凝胶LH-20上进行色谱分析。用14C标记胞嘧啶、腺嘌呤或鸟嘌呤在体外制备的RNA，用非放射性的N-AcO-AAS处理，然后进行类似的消化和色谱分析。通过这种方法，许多由核苷反应产生的加合物被证明是由核酸与同一种致癌物反应产生的，还有一些产物与单体烷基化产物不匹配。在用放射性N-AcO-AAS处理的RNA消化物中检测到标记的1-（4-乙酰氨基苯基）-2-苯基-1,2-乙二醇，这表明发生了磷酸烷基化。
