Heidemann P H, Wittliff J L
Clin Chem. 1979 Apr;25(4):622-5.
Preparation of cytosols from several estrogen target organs was compared, with use of the Beckman Airfuge and a preparative ultracentrifuge. The specific estrogen-binding capacities of cytosols prepared with these instruments were indistinguishable. Similarly, the sedimentation profiles of estrogen-receptor species as determined by sucrose gradient centrifugation were identical. We conclude that the Airfuge provides a rapid, accurate, and inexpensive means of preparing cytosols for measurements of steroid receptors in the clinical laboratory.
使用贝克曼空气离心机和制备型超速离心机比较了从几个雌激素靶器官制备胞质溶胶的情况。用这些仪器制备的胞质溶胶的特异性雌激素结合能力没有差别。同样，通过蔗糖梯度离心法测定的雌激素受体种类的沉降曲线也是相同的。我们得出结论，空气离心机为临床实验室制备用于类固醇受体测量的胞质溶胶提供了一种快速、准确且廉价的方法。
