Thompson J A, Haven M C, Langdon S M, Haven G T
Am J Clin Pathol. 1980 Mar;73(3):340-4. doi: 10.1093/ajcp/73.3.340.
Estrogen receptor has been used in determining the efficacy of endocrine therapy in mammary tumors. Complete study of a tumor usually requires two procedures, a dextran-coated charcoal method for measuring binding capacity and binding affinity and a sucrose density procedure for confirmation of 8S protein, both of which are time consuming and expensive. A stability study of the 8S and 4S molecular forms in frozen cytosols at 24 hours, 48 hours, and one week was conducted. Some loss of these, forms after storage was evident, but in all cytosols studied, the two distinct forms could still be identified. Using a group of 100 tumor samples analyzed by both methods, practical criteria for determining when the 8S molecular form should be confirmed by a sucrose density gradient on the frozen cytosol have been formulated.
雌激素受体已被用于确定内分泌疗法对乳腺肿瘤的疗效。对肿瘤进行全面研究通常需要两个步骤，一是用葡聚糖包被活性炭法测量结合容量和结合亲和力，二是用蔗糖密度法确认8S蛋白，这两种方法都既耗时又昂贵。对冷冻细胞溶质中8S和4S分子形式在24小时、48小时和一周时进行了稳定性研究。储存后这些形式出现了一些损失，但在所研究的所有细胞溶质中，仍可识别出两种不同的形式。通过对一组100个肿瘤样本采用两种方法进行分析，已制定出实用标准，以确定何时应通过对冷冻细胞溶质进行蔗糖密度梯度法来确认8S分子形式。
