Kudo K, Kikuchi M, Ishida N
Antimicrob Agents Chemother. 1972 Apr;1(4):289-95. doi: 10.1128/AAC.1.4.289.
A study of the biogenesis of the antitumor protein antibiotic neocarzinostatin (NCS) was undertaken. The production of NCS, as well as the growth of Streptomyces carzinostaticus in a production medium, was sensitive to puromycin, chloramphenicol, and actinomycin D. However, when a 12-hr culture in production medium was transferred to a nongrowth medium consisting of a phosphate buffer with Mg(2+) and Ca(2+), rapid NCS synthesis and liberation occurred. NCS production in this medium was no longer sensitive to actinomycin D, but was sensitive to puromycin and chloramphenicol. The conversion of a precursor NCS to an active form was shown to occur in this medium. Subcellular analysis suggested that NCS synthesis occurred by a mechanism similar to that of protein synthesis by membrane polysomes.
开展了一项关于抗肿瘤蛋白抗生素新制癌菌素（NCS）生物合成的研究。NCS的产生以及制癌链霉菌在生产培养基中的生长对嘌呤霉素、氯霉素和放线菌素D敏感。然而，当在生产培养基中培养12小时的培养物转移到由含Mg(2+)和Ca(2+)的磷酸盐缓冲液组成的非生长培养基中时，NCS会快速合成并释放。该培养基中NCS的产生不再对放线菌素D敏感，但对嘌呤霉素和氯霉素敏感。在此培养基中，前体NCS转化为活性形式。亚细胞分析表明，NCS的合成机制类似于膜多核糖体合成蛋白质的机制。
