Circadian changes in adrenal dopamine-beta-hydroxylase activity: dependency of change at darkness onset, and the effect of pinealectomy, on animal strain and age.

In a previous study we found a daily darkness- and pineal-dependent rise in adrenal dopamine-beta-hydroxylase (DBH) activity in 4-month-old S1 rats. The present investigation shows that aged (12 to 14-month-old) S1 rats do not have such a change in DBH activity. Similarly in the relatively younger (100-day-old) rats of a different (Holtzman) strain such a change was not found. Also pinealectomy did not seem to have any effect on adrenal DBH in either of these two strains of rats, young or old at times just before and after the onset of darkness. In both S1 and Holtzman rats, the concentration of copper-sensitive endogenous inhibitors of DBH remained unaltered following the surgical extirpation of pineal. Photoperiodic change from light to dark also had no significant effect on the inhibitor content in these two strains of rats. It is concluded that there are age and strain dependencies in the behavior of adrenal DBH activity at the daily onset of darkness.