6-磷酸葡萄糖酸脱氢酶与盘基网柄菌中尿苷二磷酸葡萄糖焦磷酸化酶的测定
6-phosphogluconate dehydrogenase and the assay of uridine diphosphate glucose pyrophosphorylase in the cellular slime mould Dictyostelium discoideum.
作者信息
Edmundson T D, Ashworth J M
出版信息
Biochem J. 1972 Feb;126(3):593-600. doi: 10.1042/bj1260593.
Abstract
- 6-Phosphogluconate dehydrogenase activity is present in all morphogenetic stages during cell differentiation in the cellular slime mould. 2. The different ratios of 6-phosphogluconate dehydrogenase/UDP-glucose pyrophosphorylase observed during this process can render spectrophotometric assays of UDP-glucose pyrophosphorylase inaccurate. 3. The disputed occurrence of increases in specific activity of UDP-glucose pyrophosphorylase during cell differentiation in the cellular slime mould is discussed in the light of these observations.
摘要
- 在细胞黏菌细胞分化的所有形态发生阶段均存在6-磷酸葡萄糖酸脱氢酶活性。2. 在此过程中观察到的6-磷酸葡萄糖酸脱氢酶与UDP-葡萄糖焦磷酸化酶的不同比率，可能会使UDP-葡萄糖焦磷酸化酶的分光光度测定不准确。3. 根据这些观察结果，讨论了细胞黏菌细胞分化过程中UDP-葡萄糖焦磷酸化酶比活性增加这一存在争议的现象。
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Uridine diphosphoglucose biosynthesis during differentiation in the cellular slime mold. II. In vitro measurements.细胞黏菌分化过程中的尿苷二磷酸葡萄糖生物合成。II. 体外测量
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Uridine diphosphoglucose biosynthesis during differentiation in the cellular slime mold. I. In vivo measurements.细胞黏菌分化过程中尿苷二磷酸葡萄糖的生物合成。I. 体内测量
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