Parfenov V N
Eur J Cell Biol. 1979 Jun;19(2):102-8.
The organization of the nucleus in the oocytes of Rana ridibunda was examined during late diplotene at the light and electron microscopic level. At this stage the chromosomes are relatively condensed and assembled in the centre of the nucleus, constituting a karyosphere. The chromosomes here are associated with the central "protein sphere" (15--20 microns in diameter), obviously at their telomeres. Numerous nucleoli are accumulated around the chromosomes, forming a karyosphere capsule and contain segregated fibrillar and granular components; structures resembling perinucleolar chromatin and fibrillar bodies (spherules) are associated with the nucleoli. Granules 30 to 40 nm in diameter are seen to surround the fibrillar spherules. "Nucleolus-like bodies" consisting of granules 10 to 15 nm in diameter which are embedded in finely fibrillar material are often associated in contact with the chromosomes. The central sphere is an accumulation of annular structures similar to those of the pore complexes of the nuclear envelope. These structures are bound to the chromosome material, the "nucleolus-like bodies" and the fibrillar bodies. A participation of "nucleolus-like bodies" in the formation of the central sphere is suggested. A possible role of the nuclear protein matrix in the construction of the karyosphere elements is discussed.
在光镜和电镜水平下，对林蛙卵母细胞双线期末期的细胞核组织进行了研究。在此阶段，染色体相对浓缩并聚集在细胞核中央，形成一个核球。这里的染色体与中央的“蛋白质球”（直径15 - 20微米）相关联，明显是在其端粒处。大量核仁聚集在染色体周围，形成一个核球囊，并包含分离的纤维状和颗粒状成分；类似核仁周围染色质和纤维体（小球）的结构与核仁相关联。可见直径30至40纳米的颗粒围绕着纤维状小球。由直径10至15纳米的颗粒嵌入精细纤维状物质组成的“类核仁体”常与染色体接触。中央球是类似于核膜孔复合体的环形结构的聚集。这些结构与染色体物质、“类核仁体”和纤维体相连。提示“类核仁体”参与中央球的形成。讨论了核蛋白基质在核球成分构建中的可能作用。
