Parfenov V N, Pochukalina G N, Gruzova M N
Tsitologiia. 1983 Nov;25(11):1243-51.
Morphological peculiarities of the oocyte nuclear organization were examined in R. ridibunda during winter and spring (February-March). Numerous nucleoli were seen to be assembled around regressive lampbrush chromosomes in the centre of the nucleus, and a central body was formed to which the chromosomes were attached. As result, a structural complex is constituted that involves a karyosphere and a capsule. Nucleoli are characterized by segregation and intensive fragmentation of their material. In result, a considerable part of nucleolar DNA is eliminated in the form of ring and polymorphous structures (micronucleoli). Besides the membranous component of nucleoli (nucleolar threads or tails) is lost. Towards the end of this period, nucleoli with complicated morphology become spherical again. The formation of the central body is started from the appearance of some small optically-light protein structures 5-20 nm in diameter (central body precursors-CBP). CBP are closely surrounded with ring micronucleoli to make intimate contact with the chromosomes and nucleolar threads. CBP commonly lie in one region of the nucleus not far from each other. The formation of a definitive central body obviously occurs due to a fusion of some small CBP. A conclusion is made of the nucleolar origin of the ring and polymorphous structures and of their essential role in the central body formation. The participation of chromosomal and eliminated nucleolar DNA in this process is discussed.
在冬季和春季（2月至3月）对湖蛙卵母细胞核组织的形态学特征进行了研究。在细胞核中央，可见大量核仁围绕着退化的灯刷染色体聚集，并且形成了一个与染色体相连的中心体。结果，构成了一个包含核球和包膜的结构复合体。核仁的特征是其物质的分离和强烈碎片化。结果，相当一部分核仁DNA以环状和多态结构（微核仁）的形式被消除。此外，核仁的膜状成分（核仁丝或尾）也消失了。在这个时期接近尾声时，形态复杂的核仁再次变成球形。中心体的形成始于一些直径为5-20纳米的小的光学浅色蛋白质结构（中心体前体-CBP）的出现。CBP被环状微核仁紧密包围，与染色体和核仁丝紧密接触。CBP通常位于细胞核的一个区域，彼此相距不远。明确的中心体的形成显然是由于一些小的CBP融合而成。得出了环状和多态结构的核仁起源及其在中心体形成中的重要作用的结论。讨论了染色体和被消除的核仁DNA在这一过程中的参与情况。
