Hamada S H
Exp Cell Biol. 1979;47(4):294-306. doi: 10.1159/000162948.
Time-lapse and electron microscopic observations were made on both epithelial and mesenchymal cells during the reassembly of embryos from dissociated cells of Strongylocentrotus purpuratus. In epithelial cells, where lysosomes are produced through the fusion of saccules formed from Golgi bodies, both phagocytosis of cell debris and resorption of differentiated cell structures were observed. In these cells, the lysosomes migrate and fuse with both autosomes and phagosomes. On the other hand, in the mesenchyme cells, where lysosomes are produced through the direct enlargement of the Golgi body's cisterna, neither phagocytosis nor resorption was observed. The migration of the lysosomes to the epithelial cell margins is the first indication of a re-establishment of cellular polarity after dissociation.
在将紫海胆离体细胞重组成胚胎的过程中，对上皮细胞和间充质细胞进行了延时和电子显微镜观察。在上皮细胞中，溶酶体是通过高尔基体形成的小囊泡融合产生的，观察到了细胞碎片的吞噬作用和分化细胞结构的吸收。在这些细胞中，溶酶体迁移并与常染色体和吞噬体融合。另一方面，在间充质细胞中，溶酶体是通过高尔基体扁平囊的直接扩大产生的，未观察到吞噬作用和吸收现象。溶酶体迁移到上皮细胞边缘是解离后细胞极性重新建立的第一个迹象。
