Hamada S H
Exp Cell Biol. 1978;46(5):310-24. doi: 10.1159/000162907.
Disaggregated single cells from the embryos of Strongylocentrotus purpuratus were allowed to reaggregate and reform quasi-normal embryos in culture. In this investigation, emphasis was placed on cell migration and its role during cell segregation. Epithelial cell migration appears to be initiated through the sequential formation of (a) cytoplasmic blebs, (b) microvilli, (c) filopodia and (d) hyaloplasmic lamellae. After functioning as a locomotive structure during cell migration, the lamella enlarges and forms a hyaloplasmic sheet, which makes up part of the blastocoel wall. The blastocoel wall is the first recognizable structure reformed in the reassembling embryo.
将紫海胆胚胎中的单细胞解离，使其在培养中重新聚集并形成类似正常的胚胎。在这项研究中，重点放在了细胞迁移及其在细胞分离过程中的作用上。上皮细胞迁移似乎是通过以下结构的依次形成而启动的：（a）细胞质泡、（b）微绒毛、（c）丝状伪足和（d）透明质层。在细胞迁移过程中作为运动结构发挥作用后，透明质层会扩大并形成透明质片，它构成了囊胚腔壁的一部分。囊胚腔壁是重组胚胎中第一个可识别的重新形成的结构。
