角膜各组成层的氧消耗

Oxygen consumption by the component layers of the cornea.

作者信息

Freeman R D

出版信息

J Physiol. 1972 Aug;225(1):15-32. doi: 10.1113/jphysiol.1972.sp009927.

DOI:10.1113/jphysiol.1972.sp009927

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1331092/

Abstract

The structural integrity of the cornea is maintained by an active fluid transport system which depends on metabolism. Experiments were designed to establish the respiratory activity of each corneal layer.2. A rapid micropolarographic technique was used to determine the oxygen consumptions of the individual layers of the rabbit cornea.3. Inherent problems of the determinations have been made minimal by the use of both denuded and whole corneal tissue preparations. Four independent measures were obtained for each limiting layer (epithelium and endothelium).4. Results show that the endothelium, epithelium and stroma use 21, 40 and 39% respectively of the total oxygen consumption of the cornea. On the basis of volumes of oxygen per unit volume tissue, epithelial oxygen utilization is about ten times that of the stroma and approximately 0.2 that of the endothelium.5. The endothelium has a larger oxygen uptake than previously reported.6. The present results, in conjunction with other studies, indicate that the ratio of glycolytic to oxidative activity in the rabbit cornea is 0.87:0.13.

摘要

角膜的结构完整性由一个依赖新陈代谢的活跃液体运输系统维持。设计实验以确定角膜各层的呼吸活性。
采用快速微极谱技术测定兔角膜各层的耗氧量。
通过使用裸露的和完整的角膜组织制剂，测定过程中固有的问题已降至最低。对每个界限层（上皮和内皮）获得了四项独立测量值。
结果表明，内皮、上皮和基质分别消耗角膜总耗氧量的21%、40%和39%。基于每单位体积组织的氧量，上皮的氧利用率约为基质的十倍，约为内皮的0.2倍。
内皮的氧摄取量比先前报道的要大。
目前的结果与其他研究相结合，表明兔角膜中糖酵解与氧化活性的比率为0.87:0.13。