Klainer A S, Perkins R L
Antimicrob Agents Chemother. 1972 Feb;1(2):164-70. doi: 10.1128/AAC.1.2.164.
Scanning electron microscopy of changes in bacteria induced by antimicrobial agents which interfere with cell wall synthesis revealed morphological alterations which correlated well with their mechanism of action. The present studies were undertaken to investigate the presence and characteristics of alterations in surface morphology resulting from the action of antibiotics known to interfere with intracellular protein synthesis. Strains of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa were exposed to kanamycin, chloramphenicol, and tobramycin during various phases of bacterial growth. A spectrum of morphological changes related to concentration of drug and duration of exposure was observed which was similar to those induced by penicillin or cephalothin. Cells were also exposed to sulfamethoxazole with similar results. The morphological abnormalities observed may be surface reflections of specific abnormalities of intracellular protein synthesis or may represent a final common pathway of druAg-induced injury at many sites within or on bacterial cells.
对抗菌剂诱导的细菌变化进行扫描电子显微镜观察，这些抗菌剂干扰细胞壁合成，结果显示形态学改变与其作用机制密切相关。本研究旨在调查已知干扰细胞内蛋白质合成的抗生素作用导致的表面形态改变的存在情况及特征。金黄色葡萄球菌、大肠杆菌和铜绿假单胞菌菌株在细菌生长的不同阶段暴露于卡那霉素、氯霉素和妥布霉素。观察到一系列与药物浓度和暴露时间相关的形态学变化，这些变化与青霉素或头孢菌素诱导的变化相似。细胞也暴露于磺胺甲恶唑，结果相似。观察到的形态学异常可能是细胞内蛋白质合成特定异常的表面反映，或者可能代表药物诱导的细菌细胞内或表面许多部位损伤的最终共同途径。
