McIntosh J R, Ogata E S, Landis S C
J Cell Biol. 1973 Feb;56(2):304-23. doi: 10.1083/jcb.56.2.304.
The axostyle of the flagellate Saccinobaculus is a motile ribbon composed of microtubules, cross-bridged to form interconnected rows. We find a centriole-related row of dark-staining tubules near the nucleus at the anterior end of the axostyle. Other tubule rows bind parallel to this primary row, acquire ordered relationships, and become the tubules of the axostyle proper. The number of tubule rows is constant in Saccinobaculus lata from the region near the nucleus to within a few micrometers of the posterior tip of the cell. In Saccinobaculus ambloaxostylus a few tubule rows are added to the axostyle posterior to the nucleus, giving this axostyle a leaf spring construction. The tubules of S. lata are held in rows by links with a 140 A periodicity along the tubule axis; bridges between rows of tubules are also seen but are not apparently periodic. Each tubule in S. ambloaxostylus shows an axial periodicity of 150 A due to pairs of arms, one of which is always part of the intrarow link. Interrow bridges in this species run either from tubule to tubule or from tubule to the free arm, but as in S. lata they do not display an obvious axial periodicity. An average unit cell is presented for the axostyle of each species, and the relation of the intertubule links to the microtubule substructure is discussed.
鞭毛虫类的腊肠菌属的轴柱是一种由微管组成的可运动的带，微管通过交叉桥连接形成相互连接的排。我们在轴柱前端靠近细胞核处发现一排与中心粒相关的深色染色微管。其他微管排与这一排初级微管平行结合，形成有序关系，并成为轴柱本身的微管。在阔腊肠菌中，从靠近细胞核的区域到细胞后尖端几微米范围内，微管排的数量是恒定的。在侧轴腊肠菌中，在细胞核后方的轴柱上增加了几排微管，使该轴柱具有板簧结构。阔腊肠菌的微管通过沿微管轴具有140埃周期的连接保持成排；微管排之间的桥也可见，但显然没有周期性。由于成对的臂，侧轴腊肠菌中的每个微管都显示出150埃的轴向周期，其中一对臂总是行内连接的一部分。该物种中的排间桥要么从微管到微管，要么从微管到自由臂，但与阔腊肠菌一样，它们没有明显的轴向周期性。给出了每个物种轴柱的平均晶胞，并讨论了微管间连接与微管亚结构的关系。
