在量热系统中应用纤维包裹酶监测代谢物。I——葡萄糖和尿素的测定。

Monitoring of metabolites applying fibre-entrapped enzymes in a calorimetric system. I -- Glucose and urea determination.

作者信息

Marconi W, Bartoli F, Morisi F, Pittalis F

出版信息

Int J Artif Organs. 1979 May;2(3):159-62.

Abstract

A simple device, consisting of a heat sensor placed in close contact to fibres containing enzymes and connected to a temperature measuring unit has been developed. The system monitors the temperature variations due to the enzymatic reaction when substrate solutions flow through the measuring cell. Fibres containing the enzymes glucose oxidase and catalase for the determination of glucose and fibres containing urease for the determination of urea were tested. A linear relationship between the substrate concentration and the deltaT recorded was obtained in both cases.

摘要

一种简单的装置已被开发出来，它由一个与含有酶的纤维紧密接触并连接到温度测量单元的热传感器组成。当底物溶液流经测量池时，该系统监测由于酶促反应引起的温度变化。测试了用于测定葡萄糖的含有葡萄糖氧化酶和过氧化氢酶的纤维以及用于测定尿素的含有脲酶的纤维。在这两种情况下，都获得了底物浓度与记录的ΔT之间的线性关系。