用于在具有固定化酶的流动系统中测定底物的微量热法。
Microcalorimetric methods for substrate determination in flow systems with immobilized enzymes.
作者信息
Schmidt H L, Krisam G, Grenner G
出版信息
Biochim Biophys Acta. 1976 Mar 11;429(1):283-90. doi: 10.1016/0005-2744(76)90051-6.
The enthalpy of processes catalyzed by immobilized enzymes in the reaction cell of a LKB-flow calorimeter is used for determination of urea (0.5-5 mumol) and glucose (0.03-0.5 mumol). Accuracy is 2-5% and the time needed for one analysis is 20 min. A sensitive "enzyme thermistor" consisting of a flow through cell with an immobilized enzyme and two thermistors is described, which permits glucose determinations (0.05-1 mumol +/- 0.03 mumol) by means of temperature difference caused by reaction heat. Coupling of enzyme reactions for increasing reaction heat and consequently sensitivity in calorimetric determinations is demonstrated.
LKB流动量热计反应池中固定化酶催化的过程的焓，用于测定尿素（0.5 - 5微摩尔）和葡萄糖（0.03 - 0.5微摩尔）。准确度为2 - 5%，一次分析所需时间为20分钟。描述了一种灵敏的“酶热敏电阻”，它由一个装有固定化酶的流通池和两个热敏电阻组成，可通过反应热引起的温差测定葡萄糖（0.05 - 1微摩尔±0.03微摩尔）。证明了酶反应的偶联可增加反应热，从而提高量热测定的灵敏度。
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