对形成玫瑰花结细胞上抗原结合位点的扫描电镜和透射电镜研究。
A scanning and transmission electron microscope study of antigen-binding sites on rosette-forming cells.
作者信息
Gudat F G, Villiger W
出版信息
J Exp Med. 1973 Feb 1;137(2):483-93. doi: 10.1084/jem.137.2.483.
Abstract
The ultrastructure of binding sites in rosette-forming cells of mice after immunization with sheep red cells was studied by means of scanning and transmission electron microscopy. It was found that the red cells were bound to the lymphocyte surface in circumscribed, immunoglobulin-containing areas, consistent with a spotlike or patchy distribution of antigen-binding immunoglobulin receptors. In these contact areas the cell membranes formed a gap of 80 A (range 75-90 A) which exhibited electron-opaque bridges at high magnification. These results are discussed in the light of the recent recognition of the formation of immunoglobulin spots on the lymphocyte surface after antigen contact. Morphological details suggest that the same mechanism is operating in rosette formation, possibly including the movement of the contact areas on the lymphocyte membrane.
摘要
用扫描电子显微镜和透射电子显微镜研究了用绵羊红细胞免疫后的小鼠玫瑰花结形成细胞中结合位点的超微结构。发现红细胞在外周含免疫球蛋白的区域与淋巴细胞表面结合，这与抗原结合免疫球蛋白受体的点状或斑状分布一致。在这些接触区域，细胞膜形成了一个80埃（范围75 - 90埃）的间隙，在高倍放大下呈现出电子不透明的桥。根据最近对抗原接触后淋巴细胞表面免疫球蛋白斑点形成的认识，对这些结果进行了讨论。形态学细节表明，玫瑰花结形成中起作用的是相同机制，可能包括淋巴细胞膜上接触区域的移动。
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本文引用的文献
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A SIMPLE METHOD FOR DETECTING SINGLE ANTIBODY-FORMING CELLS.一种检测单个抗体形成细胞的简单方法。
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[IMMUNO-CYTO-ADHESION: A SIMPLE AND QUANTITATIVE METHOD FOR THE STUDY IN VITRO OF ANTIBODY-PRODUCING CELLS].[免疫细胞黏附：一种用于体外研究抗体产生细胞的简单定量方法]
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Ultrastructure of rosette-forming cells in the mouse during the antibody response.抗体应答期间小鼠中玫瑰花结形成细胞的超微结构
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Studies on antibody-producing cells. I. Ultrastructure of 19S and 7S antibody-producing cells.抗体产生细胞的研究。I. 19S和7S抗体产生细胞的超微结构。
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Science. 1970 Oct 23;170(3956):446-7. doi: 10.1126/science.170.3956.446.
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