Ashman R F, Raff M C
J Exp Med. 1973 Jan 1;137(1):69-84. doi: 10.1084/jem.137.1.69.
Anti-thetaAKR antibody conjugated to fluorescein has been used in direct immunofluorescence tests to identify spleen theta(+) (T) sheep erythrocyte rosette-forming cells in AKR mice. Specificity studies involving A and cogenic A/thetaAKR mice clearly demonstrated that the cell surface fluorescence and cytotoxicity produced by the antiserum is directed solely toward the thetaAKR alloantigen. Approximately (3/8) of rosette-forming and non-rosette-forming spleen cells were found to be theta(+). The tendency for T cells to bind less antigen and the tendency for antigen-binding T cells to bear less theta than other spleen T cells, first suggested by other studies involving rosette-elimination by anti-thetaC3H plus complement, were confirmed by direct immunofluorescence. All AKR rosettes are specifically inhibitable by anti-immunoglobulin, including T rosettes. Antigen-induced redistribution of T cell receptors, analogous to that previously described for B cell receptors (16), occurs as readily in theta(+)RFC as in theta(-) RFC, without altering the symmetrical ring distribution of thetaAKR antigen.
与荧光素偶联的抗θAKR抗体已用于直接免疫荧光试验，以鉴定AKR小鼠脾脏中形成θ(+)（T）绵羊红细胞花环的细胞。涉及A和同基因A/θAKR小鼠的特异性研究清楚地表明，抗血清产生的细胞表面荧光和细胞毒性仅针对θAKR同种抗原。大约（3/8）的形成花环和未形成花环的脾细胞被发现是θ(+)。其他涉及用抗θC3H加补体消除花环的研究首先提出，T细胞结合较少抗原的倾向以及抗原结合T细胞比其他脾T细胞携带较少θ的倾向，通过直接免疫荧光得到了证实。所有AKR花环都可被抗免疫球蛋白特异性抑制，包括T花环。抗原诱导的T细胞受体重新分布，类似于先前描述的B细胞受体（16），在θ(+)RFC和θ(-)RFC中同样容易发生，而不会改变θAKR抗原的对称环分布。
