Oh E, Pierschbacher M, Ruoslahti E
Proc Natl Acad Sci U S A. 1981 May;78(5):3218-21. doi: 10.1073/pnas.78.5.3218.
Tissue distribution of human fibronectin injected intravenously into mice was studied by using immunofluorescence with species-specific antisera to human and mouse fibronectins. Human fibronectin was detected in the tissues of mice injected with human fibronectin. The distribution of the injected fibronectin was indistinguishable from that of mouse fibronectin. The staining for human fibronectin in the livers of injected mice was unaffected by perfusion of the livers prior to the preparation of tissue sections, and human IgG injected in mice as a control was not detectable in tissues by immunofluorescence. Mice were also injected with fragments of fibronectin with molecular weights close to 200,000. These fragments lack parts of the NH2 and COOH termini of the fibronectin polypeptide, including the part with the interchain disulfide bond(s), but retain the ability to bind to collagen and heparin and the ability to mediate cell attachment. They showed essentially no tissue incorporation, suggesting that the integrity of the fibronectin molecule is important for the accumulation of fibronectin in tissues. The incorporation of injected fibronectin into tissues demonstrated here suggests that circulating fibronectin contributes to the extracellular matrix of tissues.
通过使用针对人及小鼠纤连蛋白的种属特异性抗血清进行免疫荧光研究，对静脉注射到人小鼠体内的人纤连蛋白的组织分布情况展开了研究。在注射了人纤连蛋白的小鼠组织中检测到了人纤连蛋白。所注射的纤连蛋白的分布情况与小鼠纤连蛋白的分布并无差异。在制备组织切片之前对注射小鼠的肝脏进行灌注，并未影响注射小鼠肝脏中人纤连蛋白的染色情况，并且作为对照注射到小鼠体内的人IgG在组织中通过免疫荧光无法检测到。还向小鼠注射了分子量接近200,000的纤连蛋白片段。这些片段缺少纤连蛋白多肽的部分NH2和COOH末端，包括具有链间二硫键的部分，但保留了与胶原蛋白和肝素结合的能力以及介导细胞附着的能力。它们基本上没有组织掺入现象，这表明纤连蛋白分子的完整性对于纤连蛋白在组织中的积累很重要。此处所展示的注射纤连蛋白掺入组织的情况表明，循环中的纤连蛋白有助于组织的细胞外基质形成。
