颗粒计数免疫测定法（PACIA）。III. 通过抑制小鼠血清的凝集因子自动测定循环免疫复合物。

Particle counting immunoassay (PACIA). III. Automated determination of circulating immune complexes by inhibition of an agglutinating factor of mouse serum.

作者信息

Cambiaso C L, Sindic C, Masson P L

出版信息

J Immunol Methods. 1979;28(1-2):13-23. doi: 10.1016/0022-1759(79)90323-5.

Abstract

A factor capable of agglutinating human IgG coated particles (latex) has been found in mouse serum. This factor (MAG) was used in an unpurified form to detect circulating immune complexes in the particle counting immunoassay (PACIA) system, which allows measurement of agglutination with great precision. MAG did not react with monomeric IgG, nor with reduced and alkylated aggregated IgG. It was inhibited by immune complexes in small antigen excess. Among the various subclasses of IgG, IgA and IgM, only IgG1 and IgM when coupled to Sepharose beads displayed an inhibitory activity towards MAG. That the inhibitory factors detected in serum were immune complexes or aggregated Ig was suggested by the correlation obtained with the amounts of 'heavy' IgG found in the serum samples by Ultrogel chromatography and by the polydisperse distribution of the inhibitory factors in the heavy fraction of gradient ultracentrifugation.

摘要

在小鼠血清中发现了一种能够凝集人IgG包被颗粒（乳胶）的因子。该因子（MAG）以未纯化的形式用于颗粒计数免疫分析（PACIA）系统中检测循环免疫复合物，该系统能够高精度地测量凝集反应。MAG不与单体IgG反应，也不与还原和烷基化的聚集IgG反应。它在小抗原过量时被免疫复合物抑制。在IgG、IgA和IgM的各种亚类中，只有与琼脂糖珠偶联的IgG1和IgM对MAG表现出抑制活性。血清中检测到的抑制因子是免疫复合物或聚集Ig这一点，通过与Ultrogel色谱法在血清样品中发现的“重”IgG量的相关性以及梯度超速离心重组分中抑制因子的多分散分布得到了提示。