Limet J N, Moussebois C H, Cambiaso C L, Vaerman J P, Masson P L
J Immunol Methods. 1979;28(1-2):25-32. doi: 10.1016/0022-1759(79)90324-7.
Improved performance with the latex agglutination immunoassay, PACIA, is possible when the F(ab')2 fragments of antibody are chemically coupled at their hinge region to a protein-coated latex using a new coupling reagent, N epsilon-chloroacetyl lysine N-carboxyanhydride. This reagent, whether by orientating the complete antibody molecule or its F(ab')2 fragment or by preventing antibody denaturation, increased the sensitivity of the PACIA method. The use of F(ab')2 fragments eliminated most serum interference with the test.
当使用新型偶联试剂N-ε-氯乙酰赖氨酸N-羧基酸酐将抗体的F(ab')2片段在其铰链区化学偶联到蛋白包被的乳胶上时，乳胶凝集免疫测定法（PACI A）的性能有可能得到改善。该试剂无论是通过使完整抗体分子或其F(ab')2片段定向，还是通过防止抗体变性，都提高了PACI A方法的灵敏度。使用F(ab')2片段消除了大多数血清对检测的干扰。
