缺氧心室肌的代谢与电活动

The action potential duration of anoxic guinea-pig ventricular muscle was related to ATP generated by glycolysis. In 50 mM glucose medium the action potential duration was maintained; in 5 mM glucose medium the action potential duration shortened, the glycolytic rate declined and the ATP content was reduced.2. The action potential amplitude was related to the metabolic state of the muscle but not to the intracellular sodium concentration.3. It is suggested that changes in the action potential duration and overshoot in anoxic muscle may be due to an influence of metabolism on the slow inward current.4. Anoxic muscle incubated for 8 hr in 5 mM glucose medium had an E(m) of -77.1 mV compared to -81.1 mV in fresh muscle. The calculated E(k) of anoxic muscle was -47.4 mV.5. The resting potential of anoxic muscle was separated into two components, one dependent on potassium distribution and the other on the activity of an electrogenic sodium pump.6. The electrogenic pump component was stimulated upon raising the glucose concentration of the medium or upon raising the external potassium concentration.7. The electrogenic pump component was inhibited by ouabain or by reduction of the temperature from 35 to 8 degrees C.

缺氧豚鼠心室肌的动作电位持续时间与糖酵解产生的ATP有关。在50 mM葡萄糖培养基中，动作电位持续时间得以维持；在5 mM葡萄糖培养基中，动作电位持续时间缩短，糖酵解速率下降，ATP含量降低。
动作电位幅度与肌肉的代谢状态有关，而与细胞内钠浓度无关。
提示缺氧肌肉动作电位持续时间和超射的变化可能是由于代谢对缓慢内向电流的影响。
在5 mM葡萄糖培养基中孵育8小时的缺氧肌肉，其E(m)为-77.1 mV，而新鲜肌肉为-81.1 mV。计算得出缺氧肌肉的E(k)为-47.4 mV。
缺氧肌肉的静息电位分为两个成分，一个取决于钾的分布，另一个取决于生电钠泵的活性。
当提高培养基的葡萄糖浓度或提高细胞外钾浓度时，生电泵成分会被激活。
生电泵成分会被哇巴因抑制，或因温度从35℃降至8℃而受到抑制。

Metabolism and the electrical activity of anoxic ventricular muscle.