Achterrath U, Blümcke S, Koerner S K, Yipintsoi T, Siegelman S S, Chandler P, Hagstrom J W, Torres M, Cobbah J E, Fujii P, Veith F J
J Thorac Cardiovasc Surg. 1975 Apr;69(4):510-20.
Alveolar lavages were performed repetitively on the normal and transplanted lungs of dogs that had recieved autografts or allografts without immunosuppression. One half of the lavage returns was fixed as a cytologic smear; the other half was subjected to semi-thin section or electron microscopic examination. Of the staining methods was used, the periodic acid-Schiff (PAS) and Giemsa techniques were best for differentiating and counting cells. The Ladewig technique was best for evaluating the presence and location of fibrin. After autotransplantation, the proportion of so-called alveolar marcophages increased, reached a peak in 4 to 7 days, and then returned to normal. Phagocytized fibrin increased for the first postoperative week, but not extracellular fibrin was ever observed. After allotransplantation, a progressive decrease in the proportion, size, and vacuolization of so-called alveolar macrophages was noted along with an increase in extracellular fibrin. Intracellular fibrin could be detected only up to the third day. These findings define adequate methods for preparing and staining material obtained from diagnostic alveolar lavages, and they suggest that the procedure may serve as an index of lung allograft rejection.
对接受了自体移植或同种异体移植且未进行免疫抑制的犬的正常肺和移植肺反复进行肺泡灌洗。灌洗回收液的一半固定制成细胞学涂片；另一半进行半薄切片或电子显微镜检查。在所使用的染色方法中，过碘酸 - 希夫（PAS）和吉姆萨技术最适合细胞鉴别和计数。拉德维格技术最适合评估纤维蛋白的存在和位置。自体移植后，所谓的肺泡巨噬细胞比例增加，在4至7天达到峰值，然后恢复正常。吞噬的纤维蛋白在术后第一周增加，但从未观察到细胞外纤维蛋白。同种异体移植后，所谓的肺泡巨噬细胞的比例、大小和空泡化逐渐降低，同时细胞外纤维蛋白增加。细胞内纤维蛋白仅在第三天之前可检测到。这些发现确定了从诊断性肺泡灌洗中获取材料并进行染色的适当方法，并且表明该程序可作为肺同种异体移植排斥反应的指标。
