Aréchiga H, Cabrera-Peralta C, Huberman A
J Neurobiol. 1979 Jul;10(4):409-22. doi: 10.1002/neu.480100407.
The effect of the neurodepressing hormone (NDH) was studied on different identified motoneurons in the abdominal ganglia of the crayfish Procambarus bouvieri (Ortmann). Although differences in sensitivity were apparent, all the neurons tested responded to NDH with a reduction in spontaneous firing rate, which lasted as long as NDH was present, and, depending on the concentration and time of action of the hormone, for even longer periods. NDH activity was determined in the various parts of the central nervous system of the crayfish, being highest in the eyestalk, gradually diminishing away from the eyestalk, with a cephalo-caudal gradient, being lowest in the abdominal ganglia. High levels of NDH activity were detected in the blood. After eyestalk ablation, NDH concentration steadily diminishes in the blood and central nervous system, until virtually disappearing after 4 days; from day 5 onwards, the activity is recovered up to its original levels. NDH synthesis takes place with a time constant of approximately 3 hr in cultured isolated segments of central nervous system, being highest in the eyestalk.
研究了神经抑制激素（NDH）对布氏原螯虾（Ortmann）腹神经节中不同的已鉴定运动神经元的作用。尽管敏感性存在明显差异，但所有测试的神经元对NDH的反应都是自发放电频率降低，只要NDH存在，这种降低就会持续，并且根据激素的浓度和作用时间，甚至会持续更长时间。在小龙虾中枢神经系统的各个部位测定了NDH活性，眼柄中的活性最高，从眼柄向外逐渐降低，呈头-尾梯度，在腹神经节中最低。在血液中检测到高水平的NDH活性。眼柄切除后，血液和中枢神经系统中的NDH浓度稳步下降，直到4天后几乎消失；从第5天开始，活性恢复到原来的水平。在培养的中枢神经系统离体节段中，NDH合成的时间常数约为3小时，眼柄中的合成量最高。
