Diamond R D, Bennett J E
Infect Immun. 1973 Feb;7(2):231-6. doi: 10.1128/iai.7.2.231-236.1973.
Macrophages cultured from human peripheral blood monocytes were infected with Cryptococcus neoformans in vitro. Although C. neoformans were actively ingested, there was no detectable intracellular killing by macrophages. Over 2 days or more, intracellular fungi grew more rapidly than a corresponding inoculum of extracellular cryptococci growing in tissue culture medium containing human serum. Macrophages were induced to develop into cells which appeared to be activated by morphological and phagocytic criteria. However, these activated cells did not acquire an ability to kill or inhibit intracellular growth of C. neoformans. There were no detectable differences between macrophages from normal subjects and those from cryptococcosis patients.
用人外周血单核细胞培养的巨噬细胞在体外感染新型隐球菌。尽管新型隐球菌被巨噬细胞积极摄取，但巨噬细胞未检测到细胞内杀伤作用。在2天或更长时间内，细胞内真菌的生长速度比在含人血清的组织培养基中生长的相应接种量的细胞外隐球菌更快。巨噬细胞被诱导发育成根据形态学和吞噬标准似乎被激活的细胞。然而，这些活化细胞并未获得杀死或抑制新型隐球菌细胞内生长的能力。正常受试者的巨噬细胞与隐球菌病患者的巨噬细胞之间未检测到差异。
