苏云金芽孢杆菌产生β-内毒素与葡萄糖浓度的关系
Production of -endotoxin by Bacillus thuringiensis as a function of glucose concentrations.
作者信息
Scherrer P, Lüthy P, Trumpi B
出版信息
Appl Microbiol. 1973 Apr;25(4):644-6. doi: 10.1128/am.25.4.644-646.1973.
Abstract
The insect pathogen Bacillus thuringiensis was cultured at different glucose concentrations. Size, protein content, and insecticidal activity of the parasporal inclusions, which are formed during the sporulation process, were measured. Increased glucose concentrations led to bigger crystalline inclusions with a higher content of protein and insecticidal activity. Maximum yields of protein and endotoxin were obtained in a semisynthetic medium that contained glucose concentrations of 6 to 8 g/liter.
摘要
昆虫病原体苏云金芽孢杆菌在不同葡萄糖浓度下进行培养。对芽孢形成过程中形成的伴孢晶体的大小、蛋白质含量和杀虫活性进行了测定。葡萄糖浓度的增加导致晶体更大，蛋白质含量和杀虫活性更高。在含有6至8克/升葡萄糖浓度的半合成培养基中获得了蛋白质和内毒素的最大产量。
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