Kabasawa Y, Ishii A
Jpn J Exp Med. 1979 Feb;49(1):51-7.
The physicochemical nature of the allergenic component of Dermatophagoides pteronyssinus extract was studied by gel filtration, ion exchange chromatography, isoelectrofocusing, and polyacrylamide gel electrophoresis. Fractions were assayed for allergenic activity by the direct radioallergosorbent test (RAST) (dilution method), RAST inhibition (50% inhibition method) and human skin testing. Fractions were further studied by preparative polyacrylamide gel electrophoresis and the band showing a flow rate value of 0.35 was the most potent in allergenic activity. Fractionation by isoelectrofocusing yielded three factions with allergenic activity of pI = 4.8, PI = 7.4 and pI = 10.0 Sephadex G-100 gel filtration revealed that allergenic activity was present in fractions with molecular weight ranging from 17,000-45,000 and discrete ion exchange chromatography evidenced three fractions with very high allergenic activity. In the present study we propose that the allergenic material of D.P is composed of 3 physicochemically different substances whose molecular weight lie in the 17,000 to 45,000 range.
采用凝胶过滤、离子交换色谱、等电聚焦和聚丙烯酰胺凝胶电泳等方法研究了粉尘螨提取物变应原成分的物理化学性质。通过直接放射变应原吸附试验（RAST）（稀释法）、RAST抑制试验（50%抑制法）和人体皮肤试验对各组分的变应原活性进行了测定。通过制备性聚丙烯酰胺凝胶电泳对各组分进行了进一步研究，流速值为0.35的条带变应原活性最强。等电聚焦分级分离得到三个具有变应原活性的组分，其pI分别为4.8、7.4和10.0。葡聚糖G - 100凝胶过滤显示，变应原活性存在于分子量范围为17,000 - 45,000的组分中，离子交换色谱分离出三个具有非常高变应原活性的组分。在本研究中，我们提出粉尘螨的变应原物质由三种物理化学性质不同的物质组成，其分子量在17,000至45,000范围内。
