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Enzyme-linked immunosorbent assay with egg antigens of Schistosoma japonicum.

作者信息

Ishii A, Owhashi M

出版信息

Z Parasitenkd. 1982;67(3):279-87. doi: 10.1007/BF00927663.

DOI:10.1007/BF00927663
PMID:7136190
Abstract

A micromethod of the enzyme-linked immunosorbent assay (ELISA) was applied to infections with Schistosoma japonicum in humans and mice with egg antigens. In mouse infections, antibody responses were detected between 6 and 8 weeks after the cercarial challenge. In human infections, a circum oval precipitin positive group showed the highest reaction values in ELISA. ELISA with egg antigens did not show any cross reaction with sera of humans infected with intestinal parasites (Ascaris, Trichuris and hookworms). Fractionation of the egg antigen was attempted using gel filtration, ion-exchange and lectin affinity chromatographies. Antigenic fractions were detected in two molecular weight groups of glycoprotein, which showed major pI value at 5-6. However, ELISA with partially purified antigen (JEA-1) did not give better sensitivity or specificity than the crude extract.

摘要

相似文献

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本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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Enzyme-linked immunosorbent assay (ELISA) for detecting IgM and IgE antibodies in human schistosomiasis.用于检测人类血吸虫病中IgM和IgE抗体的酶联免疫吸附测定（ELISA）。
Am J Trop Med Hyg. 1980 Jan;29(1):82-5. doi: 10.4269/ajtmh.1980.29.82.
5
Fractionation and characterization of allergens extracted from eggs of Schistosoma japonicum.
Int Arch Allergy Appl Immunol. 1981;64(2):146-56. doi: 10.1159/000232686.
6
Serodiagnosis of human Schistosoma mansoni infections: enhanced sensitivity and specificity in ELISA using a fraction containing S. mansoni egg antigens omega 1 and alpha 1.曼氏血吸虫感染的血清学诊断：使用含有曼氏血吸虫卵抗原ω1和α1的组分进行酶联免疫吸附测定（ELISA），可提高敏感性和特异性。
Trans R Soc Trop Med Hyg. 1981;75(1):72-9. doi: 10.1016/0035-9203(81)90014-6.
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Enzyme immunoassays for parasitic diseases.用于寄生虫病的酶免疫测定法。
Trans R Soc Trop Med Hyg. 1976;70(2):98-106. doi: 10.1016/0035-9203(76)90163-2.
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Application of the enzyme linked immunosorbent assay (ELISA) for the serodiagnosis of Schistosoma mansoni infections in St. Lucia.
Trans R Soc Trop Med Hyg. 1979;73(6):636-9. doi: 10.1016/0035-9203(79)90009-9.
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Detection of antibodies in Schistosoma japonicum infections by a micro-technique of enzyme-linked immunosorbent assay (ELISA).
Jpn J Exp Med. 1979 Aug;49(4):289-92.