Lingappa V R, Lingappa J R, Blobel G
Nature. 1979 Sep 13;281(5727):117-21. doi: 10.1038/281117a0.
Ovalbumin is shown to contain an internal rather than an amino-terminal signal sequence. This internal signal can be recovered in a tryptic fragment which comprises residues 229--276 of mature ovalbumin and contains a region of striking sequence homology to amino-terminal cleaved signals of two other oviduct secretory proteins. The isolated tryptic fragment is used as a probe to demonstrate that a signal receptor is present in membrane vesicles derived from the rough but not in those derived from the smooth endoplasmic reticulum.
已表明卵清蛋白含有一个内部而非氨基末端信号序列。这个内部信号可以在一个胰蛋白酶片段中找到，该片段包含成熟卵清蛋白的229 - 276位残基，并且与另外两种输卵管分泌蛋白的氨基末端切割信号存在显著的序列同源区域。分离出的胰蛋白酶片段用作探针，以证明信号受体存在于源自糙面内质网而非滑面内质网的膜泡中。
