直肠癌三种不同肝转移灶中癌胚抗原（CEA）纯化效果的差异。
Differences in the purification effect of carcinoembryonic antigen (CEA) from the three different hepatic metastases of rectum carcinoma.
作者信息
Zawadzka H, Lisowska E, Harłozińska A, Albert Z, Richter R, Janusz M
出版信息
Neoplasma. 1979;26(2):157-67.
Carcinoembryonic actigen (CEA) was purified from three liver metastases of carcinoma recti by a conventional procedure involving perchloric acid extraction of tumor tissue and fractionation of the extract by gel filtrations on Sepharose 4B and Sephadex G-200. The preparations obtained showed a different degree of heterogeneity in SDS-polyacrylamide gel electrophoresis and crossed immunoelectrophoresis, and also a different antigenic activity. One of preparations obtained was a homogeneous CEA by all these criteria, and two others showed heterogeneity and lower antigenic activity. The most heterogeneous CEA preparation was further purified by the affinity chromatography on Concanavalin A-Sepharose 4B and by DEAE-Sephadex A-50 chromatography and the usefulness of these procedures for purification of CEA is compared.
癌胚抗原（CEA）是从三例直肠癌肝转移灶中通过常规方法纯化得到的。该方法包括用高氯酸提取肿瘤组织，并通过在琼脂糖4B和葡聚糖凝胶G - 200上进行凝胶过滤对提取物进行分级分离。所获得的制剂在十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳和交叉免疫电泳中显示出不同程度的异质性，并且抗原活性也不同。根据所有这些标准，所获得的制剂之一是均一的CEA，另外两种则表现出异质性且抗原活性较低。通过伴刀豆球蛋白A - 琼脂糖4B亲和层析和二乙氨基乙基 - 葡聚糖凝胶A - 50层析对最具异质性的CEA制剂进行了进一步纯化，并比较了这些方法对CEA纯化的有效性。
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