Kuhrt M, Pate J L
J Bacteriol. 1973 Jun;114(3):1309-18. doi: 10.1128/jb.114.3.1309-1318.1973.
Tubular structures are released from cells of Cytophaga columnaris after lysis of the cells. To determine the nature of these tubules, they were purified and their composition was determined. Tubules were isolated after treating cell lysates with 1.0% sodium dodecyl sulfate at pH 8.1, which solubilizes all structural components except tubules. Plasma membranes from the same organism were isolated by discontinuous sucrose gradient centrifugation of lysed cells. Both tubules and membranes are composed of lipids and proteins. Lipids extracted from tubules and plasma membranes produced similar patterns when examined by thin-layer chromatography. Proteins solubilized from membranes were separated into 14 bands by polyacrylamide gel electrophoresis, whereas those solubilized from tubules separated into only 5 bands. The presence of lipids in tubules from C. columnaris supports the idea that they are derived from membranes of intact cells. In this respect they are similar to tubules produced by cells of Clostridium botulinum and different from other tubular structures ("rhapidosomes") found in cells of Saprospira grandis.
柱状噬纤维菌的细胞裂解后，管状结构从细胞中释放出来。为了确定这些小管的性质，对它们进行了纯化并测定了其组成。用1.0%十二烷基硫酸钠在pH 8.1条件下处理细胞裂解物后分离出小管，该条件可溶解除小管外的所有结构成分。通过对裂解细胞进行不连续蔗糖梯度离心，从同一生物体中分离出质膜。小管和质膜均由脂质和蛋白质组成。当通过薄层色谱法检测时，从小管和质膜中提取的脂质产生了相似的图谱。通过聚丙烯酰胺凝胶电泳，从质膜中溶解的蛋白质被分离成14条带，而从小管中溶解的蛋白质仅被分离成5条带。柱状噬纤维菌小管中脂质的存在支持了它们源自完整细胞膜的观点。在这方面，它们类似于肉毒梭菌细胞产生的小管，与大腐螺旋体细胞中发现的其他管状结构（“速殖体”）不同。
