根瘤菌噬菌体DNA长度及部分变性的电子显微镜研究
Electron microscopy study of length and partial denaturation of Rhizobium bacteriophage DNA.
作者信息
Mayer F, Lotz W, Lang D
出版信息
J Virol. 1973 Jun;11(6):946-52. doi: 10.1128/JVI.11.6.946-952.1973.
Abstract
By electron microscopy and melting of the DNA of some bacteriophages from the soil bacterium Rhizobium lupini, it was found that molecular weights range between 27 and 50 Mdaltons and GC contents between 53 and 62%. All DNAs studied are linear; one has exposed single-stranded terminals. Partial heat denaturation of two phage DNA permitted mapping of AT-rich sites within unique nucleotide sequences. The maps are asymmetric with respect to the midpoints of the DNA.
摘要
通过电子显微镜观察以及对来自土壤细菌羽扇豆根瘤菌的一些噬菌体的DNA进行解链分析，发现其分子量在27至50兆道尔顿之间，GC含量在53%至62%之间。所有研究的DNA都是线性的；其中一种具有暴露的单链末端。对两种噬菌体DNA进行部分热变性，从而能够在独特的核苷酸序列中绘制富含AT的位点图谱。这些图谱相对于DNA的中点是不对称的。
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本文引用的文献
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Conjugation in starforming Rhizobium lupini.根瘤菌在形成根瘤过程中的共轭作用。
需注意的是，你提供的原文“Conjugation in starforming Rhizobium lupini.”中“starforming”表述有误，可能是“symbiotically forming”（共生形成）的错误表述，按照正确理解翻译如上。如果这不是你想要的内容，请检查原文并再次告知。
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