丝状真菌核DNA的熔解精细结构

Melting fine structure of filamentous fungus nuclear DNA.

作者信息

Szécsi A, Dobrovolszky A

出版信息

Nucleic Acids Res. 1980 Jun 11;8(11):2517-25. doi: 10.1093/nar/8.11.2517.

DOI:10.1093/nar/8.11.2517

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC324098/

Abstract

Melting fine structure of the nuclear DNA isolated from the filamentous fungus Fusarium graminearum Schwabe is presented. Optical melting profiles of nuclear DNA were analyzed by using a combination of curve fitting and derivative techniques. The "melting components" were obtained from the derivative curve by a simple decomposition technique. Differential optical melting curves of unsheared nuclear DNA indicate the presence of 15 "melting components" in filamentous fungus nuclear genome. It should be emphasized that the "melting components" observed here are different from the "thermalites" which can be observed in bacteriophage DNA. The "melting components" reported here represent the separately melting of large "blocks" of fungus nuclear DNA.

摘要

本文展示了从丝状真菌禾谷镰刀菌（Fusarium graminearum Schwabe）中分离出的核DNA的熔解精细结构。通过结合曲线拟合和导数技术分析了核DNA的光学熔解曲线。利用一种简单的分解技术从导数曲线中获得了“熔解成分”。未剪切核DNA的差示光学熔解曲线表明丝状真菌核基因组中存在15种“熔解成分”。应当强调的是，此处观察到的“熔解成分”不同于在噬菌体DNA中可观察到的“热解产物”。这里报道的“熔解成分”代表了真菌核DNA大“片段”的单独熔解。

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本文引用的文献

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Biochim Biophys Acta. 1970 Aug 8;213(2):343-51. doi: 10.1016/0005-2787(70)90042-0.

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Melting of DNA: its study and application as a research method.DNA的熔解：作为一种研究方法的研究与应用

Biopolymers. 1970 Nov;9(11):1253-306. doi: 10.1002/bip.1970.360091102.

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Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms.DNA中的重复序列。数以十万计的DNA序列拷贝已被纳入高等生物的基因组中。

Science. 1968 Aug 9;161(3841):529-40. doi: 10.1126/science.161.3841.529.

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Plurimodal distribution of base composition in DNA of some higher plants.一些高等植物DNA碱基组成的多峰分布

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Electron microscopy study of length and partial denaturation of Rhizobium bacteriophage DNA.根瘤菌噬菌体DNA长度及部分变性的电子显微镜研究

J Virol. 1973 Jun;11(6):946-52. doi: 10.1128/JVI.11.6.946-952.1973.

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