Szécsi A, Dobrovolszky A
Nucleic Acids Res. 1980 Jun 11;8(11):2517-25. doi: 10.1093/nar/8.11.2517.
Melting fine structure of the nuclear DNA isolated from the filamentous fungus Fusarium graminearum Schwabe is presented. Optical melting profiles of nuclear DNA were analyzed by using a combination of curve fitting and derivative techniques. The "melting components" were obtained from the derivative curve by a simple decomposition technique. Differential optical melting curves of unsheared nuclear DNA indicate the presence of 15 "melting components" in filamentous fungus nuclear genome. It should be emphasized that the "melting components" observed here are different from the "thermalites" which can be observed in bacteriophage DNA. The "melting components" reported here represent the separately melting of large "blocks" of fungus nuclear DNA.
本文展示了从丝状真菌禾谷镰刀菌(Fusarium graminearum Schwabe)中分离出的核DNA的熔解精细结构。通过结合曲线拟合和导数技术分析了核DNA的光学熔解曲线。利用一种简单的分解技术从导数曲线中获得了“熔解成分”。未剪切核DNA的差示光学熔解曲线表明丝状真菌核基因组中存在15种“熔解成分”。应当强调的是,此处观察到的“熔解成分”不同于在噬菌体DNA中可观察到的“热解产物”。这里报道的“熔解成分”代表了真菌核DNA大“片段”的单独熔解。