Trowbridge I S, Ralph P, Bevan M J
Proc Natl Acad Sci U S A. 1975 Jan;72(1):157-61. doi: 10.1073/pnas.72.1.157.
We have selectively labeled the surface of mouse thymus-dependent (T) and thymus-independent (B) lymphocytes by means of lactoperoxidase-catalyzed iodination. Examination of the labeled proteins by electrophoresis in the presence of sodium dodecylsulfate followed by autoradiography revealed differences in the cell surface proteins of B and T cells. Proteins from labeled thymocytes, T cell lymphomas, and both normal and activated peripheral T cells give a broad band of radioactivity corresponding to at least two protein components with apparent molecular weights of 170,000 to 190,000. This band is absent from autoradiographs of iodinated B cell proteins, which instead have another band corresponding to a protein with an apparent molecular weight of about 220,000. We have shown that these T and B cell marker proteins are synthesized by the cell and are not serum components selectively bound to the cell surface. We have also established that these proteins are the major iodinated species precipitated from Nonidet P-40 cell lysates by rabbit anti-mouse lymphocyte serum, suggesting that they are the major antigens recognized by rabbit antibodies to mouse lymphocytes.
我们通过乳过氧化物酶催化碘化法对小鼠胸腺依赖性（T）淋巴细胞和非胸腺依赖性（B）淋巴细胞的表面进行了选择性标记。在十二烷基硫酸钠存在下进行电泳，随后进行放射自显影，对标记蛋白的检测揭示了B细胞和T细胞表面蛋白的差异。来自标记胸腺细胞、T细胞淋巴瘤以及正常和活化外周T细胞的蛋白呈现出一条宽的放射性条带，对应至少两种蛋白成分，其表观分子量为170,000至190,000。碘化B细胞蛋白的放射自显影片中没有这条带，而是有另一条对应表观分子量约为220,000的蛋白的条带。我们已经证明，这些T细胞和B细胞标记蛋白是由细胞合成的，而不是选择性结合到细胞表面的血清成分。我们还确定，这些蛋白是兔抗小鼠淋巴细胞血清从Nonidet P - 40细胞裂解物中沉淀出的主要碘化物质，这表明它们是兔抗小鼠淋巴细胞抗体识别的主要抗原。
