Johnson J, Reid W M, Jeffers T K
Poult Sci. 1979 Jan;58(1):37-41. doi: 10.3382/ps.0580037.
Three floor-pen trials were conducted to compare two attenuated strains of Eimeria tenella (Wis-F-96 and Wis-F-125) with the strain of E. tenella used in a commercial planned immunization program (Coccivac). Broiler cockerels were started in floor pens on new pine shavings litter. Immunizing oocysts were administered in the drinking water during the first week with total dose per bird of 175 oocysts of the control strain (Coccivac); 175, 350, or 700 oocysts of the Wis-F-125; 350 oocysts of Wis-F-96; or no oocysts (non-seeded control). The control strain was more pathogenic than the Wis-F-125 or the Wis-F-96 strains as indicated by significantly higher lesion scores 17 days after initial exposure and by loss of two birds in the control strain exposed groups. Low lesion scores and no deaths due to coccidiosis characterized the use of the Wis-F-125 strain. Flock immunity, as determined by immunity challenge, was equivalent between the control strain and the higher dosages of Wis-F-125. The Wis-F-96 strain did not adequately immunize chickens in these experiments.
进行了三项地面围栏试验，以比较柔嫩艾美耳球虫的两种弱毒株（Wis-F-96和Wis-F-125）与商业计划免疫程序（Coccivac）中使用的柔嫩艾美耳球虫毒株。肉用小公鸡开始饲养在铺有新鲜松木刨花垫料的地面围栏中。在第一周通过饮水给予免疫卵囊，对照毒株（Coccivac）每只鸡的总剂量为175个卵囊；Wis-F-125为175、350或700个卵囊；Wis-F-96为350个卵囊；或不给予卵囊（未接种对照）。初始暴露17天后，对照毒株的病变评分显著更高，且对照毒株暴露组有两只鸡死亡，这表明对照毒株比Wis-F-125或Wis-F-96毒株致病性更强。Wis-F-125毒株的特点是病变评分低且无因球虫病导致的死亡。通过免疫攻击确定，对照毒株和较高剂量的Wis-F-125之间的群体免疫力相当。在这些实验中，Wis-F-96毒株未能充分免疫鸡群。
